URB/CCD80 Antibody

Committed Cardiac Progenitors and their Signature Genes.a Schematic showing the laminin (LN) protocol that uses LN-521 + 221 as a defined extracellular matrix together with small molecule inhibitors for the differentiation of pluripotent stem cells to day 11 committed cardiac progenitors (CCPs). b Heat map of three biological replicates from bulk RNA-seq data demonstrating the canonical progenitor gene expression on days 9 and 11. Supplementary Table 1 shows the actual transcripts per million (TPM) for each gene. c Time course analysis of scRNAseq data from days 0 to 11 for known canonical gene expression: PDGFRA, GATA4, KDR, NKX2–5, ISL1, and MESP1. d Immunoblotting of canonical progenitor genes from differentiation days 7, 9, and 11. Supplementary Fig. 1 shows the quantification of each blot from five biological replicates. e Heat map of the bulk RNA-seq data using CCP signature genes at differentiation days 9 and 11. Supplementary Table 1 shows the actual TPM for each gene. f Single-cell RNA-seq data (two biological replicates) of the CCP signature gene expression in tSNE plots from days 0, 4, 7, 9, and 11. The tSNE plot at the top left shows the overall distribution of each day using different colors. g Time course analysis of scRNAseq data from days 0 to 11 for day 11 CCP gene signature markers. h Immunoblotting of CCP signature genes from differentiation days 7, 9, and 11 (5 biological replicates). Supplementary Fig. 1 shows the quantification of each blot. Comparisons between groups were performed using two-way ANOVA with Tukey post hoc analysis. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37236990), licensed under a CC-BY license. Not internally tested by R&D Systems.

Committed Cardiac Progenitors and their Signature Genes.a Schematic showing the laminin (LN) protocol that uses LN-521 + 221 as a defined extracellular matrix together with small molecule inhibitors for the differentiation of pluripotent stem cells to day 11 committed cardiac progenitors (CCPs). b Heat map of three biological replicates from bulk RNA-seq data demonstrating the canonical progenitor gene expression on days 9 and 11. Supplementary Table 1 shows the actual transcripts per million (TPM) for each gene. c Time course analysis of scRNAseq data from days 0 to 11 for known canonical gene expression: PDGFRA, GATA4, KDR, NKX2–5, ISL1, and MESP1. d Immunoblotting of canonical progenitor genes from differentiation days 7, 9, and 11. Supplementary Fig. 1 shows the quantification of each blot from five biological replicates. e Heat map of the bulk RNA-seq data using CCP signature genes at differentiation days 9 and 11. Supplementary Table 1 shows the actual TPM for each gene. f Single-cell RNA-seq data (two biological replicates) of the CCP signature gene expression in tSNE plots from days 0, 4, 7, 9, and 11. The tSNE plot at the top left shows the overall distribution of each day using different colors. g Time course analysis of scRNAseq data from days 0 to 11 for day 11 CCP gene signature markers. h Immunoblotting of CCP signature genes from differentiation days 7, 9, and 11 (5 biological replicates). Supplementary Fig. 1 shows the quantification of each blot. Comparisons between groups were performed using two-way ANOVA with Tukey post hoc analysis. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37236990), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

URB/CCD80 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human URB
  Met129-Tyr950
  Accession # Q76M96
• Specificity: Detects human URB in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CCDC80
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for URB/CCD80 Antibody

• CCDC80
• coiled-coil domain containing 80
• down-regulated by oncogenes protein 1
• DRO1
• SSG1
• Steroid-sensitive protein 1
• URB

Background

URB (upregulated in BRS-3 deficient mice) is a 150 kDa, secreted glycoprotein that belongs to the sushi-repeat-containing protein superfamily (1, 2). Sushi repeats, otherwise known as short consensus repeats (SCRs), are 60 amino acid (aa) sequences usually involved in protein-protein interaction. They are characterized by the presence of four Cys, two Pro, one Gly and one Trp (3). Human URB is synthesized as a 950 aa precursor that contains a 21 aa signal sequence and a 929 aa mature region. The mature molecule contains three extended sushi/SCR domains of approximately 140 aa each. They bear resemblance to the fifth sushi-repeat in human SPRX (4). The three lie between aa 141‑281, 615‑760, and 771‑913, respectively. Between the first and second SCR lie two amino acid-rich regions, a Thr-rich domain (aa 347‑404), and a Lys-rich domain (aa 487‑588). Three potential N-linked glycosylation sites exist in the last two SCR’s, while six potential bipartite nuclear localization signals (NLS) occur between aa 420‑780. There are two potential alternate splice forms for human URB. One is 594 aa in length, and shows a simple truncation at Ser594. This effectively removes the second and third SCRs and two bipartite NLS (5). The second is 553 aa in length and shows a simple truncation after Lys553. This eliminates four bipartite NLSs, the second and third SCRs, and part of the Lys-rich domain (6). Full-length human URB is 83%, 84% and 87% aa identical to rat, mouse and bovine URB, respectively. URB is found in chondrocytes and appears to be downregulated upon CFU-Fibroblast differentiation (1). Thus, it may play a role in skeletogenesis.

1. Liu, Y. et al. (2004) Biochem. Biophys. Res. Commun. 322:497.
2. Aoki, K. et al. (2002) Biochem. Biophys. Res. Commun. 290:1282.
3. Anatova, J. et al. (1989) Biochemistry 28:4754.
4. Meindl, A. et al. (1995) Hum. Mol. Genet. 4:2339.
5. Isogai, T. et al. (2002) GenBank Accession # BAC11475.
6. Strausberg, R.L. et al. (2002) GenBank Accession # AAH86876.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for URB/CCD80 Antibody (AF3410)(3)

Publications using AF3410

• Yap L, Chong LY, Tan C et al. Pluripotent stem cell-derived committed cardiac progenitors remuscularize damaged ischemic hearts and improve their function in pigs npj Regenerative Medicine 2023-05-26 [PMID: 37236990]
• Yap L, Chong LY, Tan C et al. Pluripotent stem cell-derived committed cardiac progenitors remuscularize damaged ischemic hearts and improve their function in pigs npj Regenerative Medicine 5/26/2023 [PMID: 37236990]
• Okada T, Nishizawa H, Kurata A, Tamba S, Sonoda M, Yasui A, Kuroda Y, Hibuse T, Maeda N, Kihara S, Hadama T, Tobita K, Akamatsu S, Maeda K, Shimomura I, Funahashi T URB is abundantly expressed in adipose tissue and dysregulated in obesity. Biochem. Biophys. Res. Commun., 2008-01-04;367(2):370-6. 2008-01-04 [PMID: 18178152] (Western Blot, Human)

Bioinformatics

• Gene Symbol: CCDC80
• Uniprot:
  • Q76M96()