UQCRFS1 Antibody (JG62-31)

Western Blot: UQCRFS1 Antibody (JG62-31) [NBP2-75697] - Analysis of UQCRFS1 on different lysates using anti-UQCRFS1 antibody at 1/1,000 dilution. Positive control: Lane 1: A549, Lane 2: 293 ,Lane 3: Mouse kidney

Immunocytochemistry/Immunofluorescence: UQCRFS1 Antibody (JG62-31) [NBP2-75697] - Staining UQCRFS1 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: UQCRFS1 Antibody (JG62-31) [NBP2-75697] - Analysis of paraffin-embedded rat skeletal muscle tissue using anti-UQCRFS1 antibody. Counter stained with hematoxylin.

Flow Cytometry: UQCRFS1 Antibody (JG62-31) [NBP2-75697] - Analysis of A549 cells with UQCRFS1 antibody at 1/100 dilution (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: UQCRFS1 Antibody (JG62-31) [NBP2-75697] - Staining UQCRFS1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: UQCRFS1 Antibody (JG62-31) [NBP2-75697] - Staining UQCRFS1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: UQCRFS1 Antibody (JG62-31) [NBP2-75697] - Analysis of paraffin-embedded human colon cancer tissue using anti-UQCRFS1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: UQCRFS1 Antibody (JG62-31) [NBP2-75697] - Analysis of paraffin-embedded human appendix tissue using anti-UQCRFS1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: UQCRFS1 Antibody (JG62-31) [NBP2-75697] - Analysis of paraffin-embedded human kidney tissue using anti-UQCRFS1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: UQCRFS1 Antibody (JG62-31) [NBP2-75697] - Analysis of paraffin-embedded mouse cerebellum tissue using anti-UQCRFS1 antibody. Counter stained with hematoxylin.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC, IP
• Clone: JG62-31
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

UQCRFS1 Antibody (JG62-31) Summary

• Description: Novus Biologicals Rabbit UQCRFS1 Antibody (JG62-31) (NBP2-75697) is a recombinant monoclonal antibody validated for use in IHC, WB, Flow, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human UQCRFS1 aa 180-274 / 274. (SwissProt: P47985 Human; SwissProt: Q9CR68 Mouse; SwissProt: P20788 Rat)
• Localization: Mitochondrion.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: UQCRFS1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:50
  • Immunocytochemistry/ Immunofluorescence 1:500-1:2000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:500-1:2000
• Theoretical MW: 21 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for UQCRFS1 Antibody (JG62-31)

• Complex III subunit 5
• Cytochrome b-c1 complex subunit 5
• cytochrome b-c1 complex subunit Rieske, mitochondrial
• EC 1.10.2.2
• Rieske iron-sulfur protein
• RIP1
• RIS1
• RISPUbiquinol-cytochrome c reductase iron-sulfur subunit
• ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1
• UQCR5

Background

RIP1 is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis Function: The transit peptide of the Rieske protein seems

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: UQCRFS1