UQCRC1 Antibody (OTI1G6) - Azide and BSA Free

Western Blot: UQCRC1 Antibody (OTI1G6) - Azide and BSA Free [NBP2-74796] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UQCRC1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UQCRC1.

Immunohistochemistry: UQCRC1 Antibody (OTI1G6) - Azide and BSA Free [NBP2-74796] - Staining of paraffin-embedded Human liver tissue using anti-UQCRC1 mouse monoclonal antibody.

Flow Cytometry: UQCRC1 Antibody (OTI1G6) - Azide and BSA Free [NBP2-74796] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-UQCRC1 antibody, and then analyzed by flow cytometry.

Western Blot: UQCRC1 Antibody (OTI1G6) - Azide and BSA Free [NBP2-74796] - Analysis of extracts (35ug) from 9 different cell lines by using g anti-UQCRC1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunohistochemistry: UQCRC1 Antibody (OTI1G6) - Azide and BSA Free [NBP2-74796] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-UQCRC1 mouse monoclonal antibody.

Immunohistochemistry: UQCRC1 Antibody (OTI1G6) - Azide and BSA Free [NBP2-74796] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-UQCRC1 mouse monoclonal antibody.

Immunohistochemistry: UQCRC1 Antibody (OTI1G6) - Azide and BSA Free [NBP2-74796] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-UQCRC1 mouse monoclonal antibody.

Immunohistochemistry: UQCRC1 Antibody (OTI1G6) - Azide and BSA Free [NBP2-74796] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-UQCRC1 mouse monoclonal antibody.

Immunohistochemistry: UQCRC1 Antibody (OTI1G6) - Azide and BSA Free [NBP2-74796] - Staining of paraffin-embedded Human Kidney tissue using anti-UQCRC1 mouse monoclonal antibody.

Immunohistochemistry: UQCRC1 Antibody (OTI1G6) - Azide and BSA Free [NBP2-74796] - Staining of paraffin-embedded Human prostate tissue using anti-UQCRC1 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt, Ca, Pm, Pm
• Applications: WB, Flow, IHC, CyTOF-ready
• Clone: OTI1G6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

UQCRC1 Antibody (OTI1G6) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse UQCRC1 Antibody (OTI1G6) - Azide and BSA Free (NBP2-03825) is a monoclonal antibody validated for use in IHC, WB and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human recombinant protein fragment corresponding to amino acids 46-305 of human UQCRC1 (NP_003356) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: UQCRC1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1000
• Theoretical MW: 52.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for UQCRC1 Antibody (OTI1G6) - Azide and BSA Free

• Complex III subunit 1
• Core protein I
• cytochrome b-c1 complex subunit 1, mitochondrial
• D3S3191
• QCR1
• ubiquinol-cytochrome c reductase core protein I
• Ubiquinol-cytochrome-c reductase complex core protein 1
• UQCR1

Background

This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. This protein may mediate formation of the complex between cytochromes c and c1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: UQCRC1