Western Blot: UNG Antibody [NBP1-49985] - Detection of Human UNG by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ...read more
The human UNG-gene encodes nuclear (UNG2) and mitochondrial (UNG1) forms of uracil-DNA glycosylase using differentially regulated promoters, PA and PB, and alternative splicing to produce two proteins with unique N-terminal sorting sequences. Uracil in DNA may result from deamination of cytosine which can give rise to transition mutations. Uracil-DNA glycosylase is the DNA repair enzyme responsible for the removal of uracil from DNA. This is probably a biologically important function in the prevention of mutagenesis resulting from cytosine deamination. UNG2 removes misincorporated dUMP residues.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
"We have validated the specificity of NBP1-49985 (lot A1) by western blot (diluted at 1:1,000) on MAGIC-5B cells 4 days after transfection with UNG2 specific siRNA.UNG2 siRNA44: 5â€™-CGCGTTCGCTGCCTCCTCAGCTCCA-3â€™From nt44 to 68 of gi 19718750 ref NM_080911.1."
Blocking Buffer: 5% milk in PBS 0.1% Tween-20, Blocking Time: 1 hour, Blocking Temp: room temperature
Secondary Ab: Anti-Rabbit HRP for UNG and anti-Mouse HRP for alpha-tubulin
Specific Bands: 37 (UNG2), 31 (UNG1), Non-Specific Bands: 35 kDa
"We have validated the specificity of NBP1-49985 (lot A1) by western blot (diluted at 1:1,000) on MAGIC-5B cells 4 days after transfection with UNG2 specific siRNA.UNG2 siRNA44: 5Ã¢â‚¬â„¢-CGCGTTCGCTGCCTCCTCAGCTCCA-3Ã¢â‚¬â„¢From nt44 to 68 of gi 19718750 ref NM_080911.1."
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