![Western Blot: UNG Antibody [NBP1-49985] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and UNG knockout (KO) HeLa cell line. PVDF membrane was probed with 1:2000 of Rabbit Anti-Human UNG Polyclonal Antibody (Catalog # NBP1-49985) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for UNG at approximately 38 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/UNG-Antibody-Knockdown-Validated-NBP1-49985-img0003.jpg "Western Blot: UNG Antibody [NBP1-49985] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and UNG knockout (KO) HeLa cell line. PVDF membrane was probed with 1:2000 of Rabbit Anti-Human UNG Polyclonal Antibody (Catalog # NBP1-49985) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for UNG at approximately 38 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IP (-), Mycoplasma |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Description | Novus Biologicals Rabbit UNG Antibody - BSA Free (NBP1-49985) is a polyclonal antibody validated for use in WB and IP. Anti-UNG Antibody: Cited in 4 publications. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | The immunogen for this product maps to a region between residue 263 and 313 of human Uracil-DNA Glycosylase using the numbering given in entry NP_550433.1 (GeneID 7374). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | UNG |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Verified Customer |
WB | Human | 12/01/2011 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for UNG Antibody (NBP1-49985)Find related products by research area.
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| Gene Symbol | UNG |
![Western Blot: UNG Antibody [NBP1-49985] - Detection of Human UNG by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-UNG antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. UNG was successfully immunoprecipitated by rabbit anti-UNG antibody which recognizes an upstream epitope. For blotting immunoprecipitated UNG was used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).](http://images.novusbio.com/fullsize/UNG-Antibody-Western-Blot-NBP1-49985-img0002.jpg "Western Blot: UNG Antibody [NBP1-49985] - Detection of Human UNG by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-UNG antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. UNG was successfully immunoprecipitated by rabbit anti-UNG antibody which recognizes an upstream epitope. For blotting immunoprecipitated UNG was used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
