UNG Antibody

Images

 
Western Blot: UNG Antibody [NBP1-49985] - Detection of Human UNG by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP (-)
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

UNG Antibody Summary

Immunogen
The immunogen for this product maps to a region between residue 263 and 313 of human Uracil-DNA Glycosylase using the numbering given in entry NP_550433.1 (GeneID 7374).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
UNG
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
Reviewed Applications
Read 1 Review rated 5
using
NBP1-49985 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for UNG Antibody

  • DGU
  • DGUEC 3.2.2
  • HIGM4EC 3.2.2.27
  • UDG
  • UDGDKFZp781L1143
  • UNG
  • UNG15
  • UNG1uracil-DNA glycosylase 1, uracil-DNA glycosylase 2
  • UNG2
  • uracil-DNA glycosylase

Background

The human UNG-gene encodes nuclear (UNG2) and mitochondrial (UNG1) forms of uracil-DNA glycosylase using differentially regulated promoters, PA and PB, and alternative splicing to produce two proteins with unique N-terminal sorting sequences. Uracil in DNA may result from deamination of cytosine which can give rise to transition mutations. Uracil-DNA glycosylase is the DNA repair enzyme responsible for the removal of uracil from DNA. This is probably a biologically important function in the prevention of mutagenesis resulting from cytosine deamination. UNG2 removes misincorporated dUMP residues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for UNG Antibody (NBP1-49985) (0)

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Review for UNG Antibody (NBP1-49985) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-49985:
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WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot UNG NBP1-49985
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reviewed by:
Anonymous
WB Human 12/01/2011
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Summary

ApplicationWestern Blot
Sample TestedWhole cell lysate, Sample Amount: 25 ug
SpeciesHuman
LotA1
Comments"We have validated the specificity of NBP1-49985 (lot A1) by western blot (diluted at 1:1,000) on MAGIC-5B cells 4 days after transfection with UNG2 specific siRNA.UNG2 siRNA44: 5’-CGCGTTCGCTGCCTCCTCAGCTCCA-3’From nt44 to 68 of gi 19718750 ref NM_080911.1."

Blocking

Blocking DetailsBlocking Buffer: 5% milk in PBS 0.1% Tween-20, Blocking Time: 1 hour, Blocking Temp: room temperature

Primary Anitbody

Dilution RatioDilution Ratio: UNG (1/1,000);tubulin (1/10,000), Incubation Dilution Buffer:blocking buffer, Incubation Time:overnight, Temp:4C

Secondary Antibody

Secondary DescriptionSecondary Ab: Anti-Rabbit HRP for UNG and anti-Mouse HRP for alpha-tubulin

Details

Detection NotesSpecific Bands: 37 (UNG2), 31 (UNG1), Non-Specific Bands: 35 kDa

Comments

Comments"We have validated the specificity of NBP1-49985 (lot A1) by western blot (diluted at 1:1,000) on MAGIC-5B cells 4 days after transfection with UNG2 specific siRNA.UNG2 siRNA44: 5’-CGCGTTCGCTGCCTCCTCAGCTCCA-3’From nt44 to 68 of gi 19718750 ref NM_080911.1."

Product General Protocols

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Video Protocols

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FAQs for UNG Antibody (NBP1-49985) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for UNG Antibody (NBP1-49985)

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Research Areas for UNG Antibody (NBP1-49985)

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Blogs on UNG

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Recent Reviews

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1
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Anonymous
12/01/2011
Application: WB
Species: Human

Bioinformatics

Gene Symbol UNG
Entrez