AGAP2 Antibody

Western Blot: AGAP2 Antibody [NBP2-24498] - Analysis of AGAP2 in Jurkat cell lysate in the 1) absence and 2) presence of immunizing peptide using this antibody. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Immunohistochemistry-Paraffin: AGAP2 Antibody [NBP2-24498] - Analysis human brain tissue using an isotype control (top left) and this antibody (bottom left, right) at 5 ug/ml.

• Reactivity: Hu, Mu, Rt, Ca, Eq, Pm
• Applications: WB, ICC/IF, IHC, IHC-P, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

AGAP2 Antibody Summary

• Immunogen: A portion of amino acids 450-500 of human AGAP2 was used as the immunogen.
• Specificity: 100% homologous to Human (isoform CRA_a).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AGAP2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Knockdown Validated
  • Western Blot 1-3 ug/ml
• Application Notes: Use in ICC/IF,KD reported in scientific literature (PMID:34411567).

Reactivity Notes

Opossum (86%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 ml PBS and 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for AGAP2 Antibody

• AGAP-2
• ArfGAP with GTPase domain, ankyrin repeat and PH domain 2
• arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2
• centaurin, gamma 1
• centaurin-gamma-1
• CENTG1
• cnt-g1
• FLJ16430
• GGAP2
• GTP-binding and GTPase activating protein 2
• GTP-binding and GTPase-activating protein 2
• KIAA0167
• Phosphatidylinositol-3-kinase enhancer
• phosphoinositide 3-kinase enhancer
• PIKEArf GAP with GTP-binding protein-like, ANK repeat and PH domains 2

Background

AGAP2 (also known as centaurin-gamma1, ARF-GAP with GTP-binding protein-like, ankyrin repeat and pleckstrin homology domains 2, Phosphatidyl-inositol-3-kinase enhancer, PIKE, GTP-binding and GTPase activating protein 2 and GGAP2) is a GTPase activating protein that inactivates Arf. The expression of AGAP2 is amplified in human glioblastoma cells. AGAP2 protein binds to and activates the protein kinase Akt and prevents apoptosis of cancer cells. AGAP2 also increases motility and invasive activity of cancer cells. AGAP2 may serve as a diagnostic and therapeutic target of human cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AGAP2 Antibody (NBP2-24498)(1)

Publication using NBP2-24498

• Sun M, Sheng H, Wu T Et al. PIKE-A promotes glioblastoma growth by driving PPP flux through increasing G6PD expression mediated by phosphorylation of STAT3 Biochemical pharmacology 2021-08-16 [PMID: 34411567] (ICC/IF, KD, WB, Human)

Bioinformatics

• Gene Symbol: AGAP2