UNC45B Antibody

Western Blot: UNC45B Antibody [NBP1-47199] - (0.3ug/ml) staining of Human Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

UNC45B Antibody Summary

• Description: Novus Biologicals Goat UNC45B Antibody (NBP1-47199) is a polyclonal antibody validated for use in WB and ELISA. Anti-UNC45B Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-RSDKLRQKIFKER corresponding to internal region according to NP_001028748.1.
• Epitope: internal region
• Specificity: This antibody is expected to recognise both reported isoforms (NP_775259.1and NP_001028748.1).
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: UNC45B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:2000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 90 kDa band observed in human skeletal muscle lysates (calculated MW of 104 kDa band according to NP_775259.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for UNC45B Antibody

• CMYA4
• FLJ38610
• MGC119540
• MGC119541
• protein unc-45 homolog B
• SMUNC45
• striated muscle UNC45
• unc-45 homolog B (C. elegans)
• UNC-45B
• UNC45cardiomyopathy associated 4

Background

UNC45B plays a role in myoblast fusion and sarcomere organization (Price et al., 2002 [PubMed 12356907]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for UNC45B Antibody (NBP1-47199)(1)

Publication using NBP1-47199

• Landsverk ML, Li S, Hutagalung AH et al. The UNC-45 chaperone mediates sarcomere assembly through myosin degradation in Caenorhabditis elegans. J Cell Biol 2007-04-23 [PMID: 17438072]

Bioinformatics

• Gene Symbol: UNC45B
• Entrez:
  • 146862(Human)
  • 217012(Mouse)
  • 303373(Rat)
• Uniprot:
  • NP_775259.1(Human)