ULBP-3 Antibody (166510) [Phycoerythrin]

PC-3 human prostate carcinoma cell line was stained with Mouse Anti-Human ULBP-3 PE-conjugated Monoclonal Antibody (Catalog # FAB1517P, filled histogram) or isotype control antibody (IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Human monocyte-derived dendritic cells were stained with Mouse Anti-Human ULBP-3 PE-conjugated Monoclonal Antibody (Catalog # FAB1517P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.

BaF3 mouse pro-B cell line transfected with human ULBP-3 was stained with Mouse Anti-Human ULBP-3 PE-conjugated Monoclonal Antibody (Catalog # FAB1517P, filled histogram) or isotype control antibody (IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Histograms showing sample flow cytometry profiles for the ligands studied. Bars in each plot indicate the population expressing each aNKR, Gates were set using Unstained, single stained controls, fluorescence minus one, and secondary antibody alone controls. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/1999-4915/9/10/295), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: Flow
• Clone: 166510
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

ULBP-3 Antibody (166510) [Phycoerythrin] Summary

• Immunogen: BaF3 mouse pro-B cell line transfected with human ULBP-3
• Specificity: Detects human ULBP-3 in ELISAs. Does not cross-react with recombinant human (rh) ULBP-1 or rhULBP-2.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: ULBP3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ULBP-3 Antibody (166510) [Phycoerythrin]

• NKG2D ligand 3
• RAET1N
• UL16 binding protein 3
• ULBP3
• ULBP-3

Background

ULBP-3 is a member of a family of cell-surface proteins that function as ligands for human NKG2D. ULBP-3 has also been described under the names RaeT1N (retinoic acid early transcript), NKG2DL3, and ALCAN-gamma . The name ULBP-3 derives from the original identification of three proteins, ULBP-1, -2, and -3, as ligands for the human cytomegalovirus glycoprotein UL16; they were designated UL16 binding proteins (ULBP). The gene for ULBP-3 resides in a cluster of ten related genes, six of which encode potentially functional glycoproteins. Amino acid sequence identity within this family ranges from 30‑60%. These proteins are distantly related to MHC class I proteins, but they possess only the alpha 1 and alpha 2 Ig-like domains, and they have no capacity to bind peptide or interact with beta 2‑microglobulin. Some family members, including ULBP-3, are anchored to the membrane via a GPI-linkage, whereas others have transmembrane domains. ULBP-3 and several other family members are known to bind to human NKG2D, an activating receptor expressed on NK cells, NKT cells, gamma δ T cells, and CD8⁺ alpha beta T cells. Engagement of NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. The ULBPs are expressed on some tumor cells and have been implicated in tumor surveillance (1‑7).

1. Cosman, D. et al. (2001) Immunity 14:123.
2. Kubin, M. et al. (2001) Eur. J. Immunol. 31:1428.
3. Sutherland, C. et al. (2002) J. Immunol. 168:671.
4. Steinle, A. et al. (2001) Immunogenetics 53:279.
5. Sutherland, C. et al. (2001) Immunol. Rev. 181:185.
6. Pende, D. et al. (2002) Cancer Res. 62:6178.
7. Radosavljevic, M. et al. (2002) Genomics 79:114.
8. NKG2D and its Ligands (2002) http://www.RnDSystems.com/.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ULBP-3 Antibody (FAB1517P)(22)

Publications using FAB1517P

• He, X;Wang, L;Zeng, W;Wang, Y;Chen, N;Yang, P;Ti, A;Zhang, Q;Shao, Y;Wang, M;Huang, Z;Zhang, X;Xu, M;Liang, L;Wang, X;Ding, X;Zhu, T;Zhang, P;Pan, Z;Yang, F;Zhou, Y;Mo, G;Hu, J;Yue, Y;Hu, J;Deng, Y;Ho, T;Church, GM;Hu, Y;Huang, H;Yang, L; Regulatable C-X-C chemokine receptor type 4 in iPSC-derived NK cells improves bone marrow chemotaxis and targeting resident tumor Trends in biotechnology 2025-04-30 [PMID: 40312161] (Human)
• Lee, S;Chae, SJ;Jang, IH;Oh, SC;Kim, SM;Lee, SY;Kim, JH;Ko, J;Kim, HJ;Song, IC;Kim, JK;Kim, TD; B7H6 is the predominant activating ligand driving natural killer cell-mediated killing in patients with liquid tumours: evidence from clinical, in silico, in vitro, and in vivo studies EBioMedicine 2024-11-22 [PMID: 39579618] (FACS, Human)
• Amanda A. van Vliet, Ella Peters, Denise Vodegel, Daniëlle Steenmans, Monica Raimo, Susan Gibbs et al. Early TRAIL-engagement elicits potent multimodal targeting of melanoma by CD34+ progenitor cell-derived NK cells iScience 2023-07-21 [PMID: 37426355]
• Koh, EK;Lee, HR;Son, WC;Park, GY;Bae, J;Park, YS; Antitumor effects of NK cells expanded by activation pre?processing of autologous feeder cells before irradiation in colorectal cancer Oncology letters 2023-06-01 [PMID: 37153058] (Flow Cytometry, Human)
• NL de Vries, J van de Haa, V Veninga, M Chalabi, ME Ijsselstei, M van der Pl, J van den Bu, D Ruano, JG van den Be, JB Haanen, LJ Zeverijn, BS Geurts, GF de Wit, TW Battaglia, H Gelderblom, HMW Verheul, TN Schumacher, LFA Wessels, F Koning, NFCC de Miranda, EE Voest gammadelta T cells are effectors of immunotherapy in cancers with HLA class I defects Nature, 2023-01-11;613(7945):743-750. 2023-01-11 [PMID: 36631610] (Flow Cytometry, Human)
• Y Murayama, Y Kasahara, N Kubo, C Shin, M Imamura, N Oike, T Ariizumi, A Saitoh, M Baba, T Miyazaki, Y Suzuki, Y Ling, S Okuda, K Mihara, A Ogose, H Kawashima, C Imai NKp44-based chimeric antigen receptor effectively redirects primary T cells against synovial sarcoma Translational Oncology, 2022-08-20;25(0):101521. 2022-08-20 [PMID: 35998437] (Flow Cytometry, Human)
• Thibault Carré, Jerome Thiery, Bassam Janji, Stéphane Terry, Gwendoline Gros, Guillaume Meurice et al. Selection of tumor‑resistant variants following sustained natural killer cell‑mediated immune stress Oncology Reports 2020-11-27 [PMID: 33416152]
• DR Makanga, F Da Rin de, G David, C Willem, L Dubreuil, N Legrand, T Guillaume, P Peterlin, A Lebourgeoi, MC Béné, A Garnier, P Chevallier, K Gendzekhad, A Cesbron, K Gagne, B Clemenceau, C Retière Genetic and Molecular Basis of Heterogeneous NK Cell Responses against Acute Leukemia Cancers (Basel), 2020-07-16;12(7):. 2020-07-16 [PMID: 32708751] (Flow Cytometry, Human)
• D Suzuki, C Flahou, N Yoshikawa, I Stirblyte, Y Hayashi, A Sawaguchi, M Akasaka, S Nakamura, N Higashi, H Xu, T Matsumoto, K Fujio, MG Manz, A Hotta, H Takizawa, K Eto, N Sugimoto iPSC-Derived Platelets Depleted of HLA Class I Are Inert to Anti-HLA Class I and Natural Killer Cell Immunity Stem Cell Reports, 2019-12-26;0(0):. 2019-12-26 [PMID: 31883921] (Flow Cytometry, Human)
• M Chollat-Na, T Ben Safta-, D Haferssas, G Meurice, S Chouaib, J Thiery The pharmalogical reactivation of p53 function improves breast tumor cell lysis by granzyme B and NK cells through induction of autophagy Cell Death Dis, 2019-09-20;10(10):695. 2019-09-20 [PMID: 31541080] (Flow Cytometry, Human)
• Y Tanaka, T Nakazawa, M Nakamura, F Nishimura, R Matsuda, K Omoto, Y Shida, T Murakami, I Nakagawa, Y Motoyama, H Morita, T Tsujimura, H Nakase Ex vivo-expanded highly purified natural killer cells in combination with temozolomide induce antitumor effects in human glioblastoma cells in vitro PLoS ONE, 2019-03-06;14(3):e0212455. 2019-03-06 [PMID: 30840664] (Flow Cytometry, Human)
• A Tremblay-M, S Coenraads, Z Kiani, FP Dupuy, NF Bernard Expression of ligands for activating natural killer cell receptors on cell lines commonly used to assess natural killer cell function BMC Immunol., 2019-01-29;20(1):8. 2019-01-29 [PMID: 30696399] (Flow Cytometry, Human)
• Eytan Breman, Benjamin Demoulin, Sophie Agaugué, Sebastien Mauën, Alexandre Michaux, Lorraine Springuel et al. Overcoming Target Driven Fratricide for T Cell Therapy Frontiers in Immunology 2018-12-12 [PMID: 30619300]
• M Najar, M Fayyad-Kaz, N Meuleman, D Bron, H Fayyad-Kaz, L Lagneaux Mesenchymal stromal cells of the bone marrow and natural killer cells: cell interactions and cross modulation J Cell Commun Signal, 2018-01-19;0(0):. 2018-01-19 [PMID: 29350342] (Flow Cytometry, Human)
• A Tremblay-M, J Bruneau, B Lebouché, I Lisovsky, R Song, NF Bernard Expression Profiles of Ligands for Activating Natural Killer Cell Receptors on HIV Infected and Uninfected CD4? T Cells Viruses, 2017-10-12;9(10):. 2017-10-12 [PMID: 29023371] (Flow Cytometry, Human)
• JRM Van Audena, J De Waele, E Marcq, J Van Loenho, E Lion, JMJ Van den Be, R Jesenofsky, A Masamune, G Roeyen, P Pauwels, F Lardon, M Peeters, ELJ Smits Interleukin-15 stimulates natural killer cell-mediated killing of both human pancreatic cancer and stellate cells Oncotarget, 2017-01-01;0(0):. 2017-01-01 [PMID: 28615524] (Flow Cytometry, Human)
• Wei-Chen Huang T Cells Infiltrating Diseased Liver Express Ligands for the NKG2D Stress Surveillance System J. Immunol, 2016-12-28;0(0):. 2016-12-28 [PMID: 28031333] (Flow Cytometry, Human)
• Combination treatment with decitabine and ionizing radiation enhances tumor cells susceptibility of T cells Sci Rep, 2016-09-27;6(0):32470. 2016-09-27 [PMID: 27671170] (Flow Cytometry, Human)
• Decitabine enhances anti-CD33 monoclonal antibody BI 836858-mediated natural killer ADCC against AML blasts Blood, 2016-03-24;127(23):2879-89. 2016-03-24 [PMID: 27013443] (Flow Cytometry, Human)
• Clark GJ et al. New insights into the phenotype of human dendritic cell populations. Clin Transl Immunology 1/1/2016 [PMID: 26900474]
• Sandra A. Jacobs, Jeroen Plessers, Jef Pinxteren, Valerie D. Roobrouck, Catherine M. Verfaillie, Stefaan W. Van Gool Mutual Interaction between Human Multipotent Adult Progenitor Cells and NK Cells Cell Transplantation 2014-09-01 [PMID: 23562064]
• Hedlund M, Nagaeva O, Kargl D, Baranov V, Mincheva-Nilsson L Thermal- and Oxidative Stress Causes Enhanced Release of NKG2D Ligand-Bearing Immunosuppressive Exosomes in Leukemia/Lymphoma T and B Cells. PLoS ONE, 2011-02-25;6(2):e16899. 2011-02-25 [PMID: 21364924] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: ULBP3