ULBP-2/5/6 Antibody [Unconjugated]

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ULBP‑2/5/6 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using 10 µg/mL Goat Anti-Human ULBP‑2/5/6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1298) ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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ULBP-2/5/6 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human ULBP-2
Gly26-Ser217
Accession # Q9BZM5
Specificity
Detects human ULBP-2, ULBP-5, and RAET1L/ULBP-6 in Western blots. In direct ELISAs and Western blots, less than 2% cross-reactivity with recombinant human (rh) ULBP-1, rhULBP-3, and rhULBP-4 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Blockade of Receptor-ligand Interaction
Publications
Read Publications using
AF1298 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ULBP-2/5/6 Antibody [Unconjugated]

  • ALCAN-alpha
  • N2DL2
  • NKG2DL2
  • RAET1H
  • RAET1L
  • UL16 binding protein 2
  • ULBP2
  • ULBP-2/5/6

Background

ULBPs activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. Binding of ULBPs ligands to NKG2D induces calcium mobilization and activation of the JAK2, STAT5, ERK and PI3K kinase/Akt signal transduction pathway. The name ULBP derives from the original identification of three proteins, ULBP-1, -2, and -3, as ligands for the human cytomegalovirus glycoprotein UL16; they were designated UL16 binding proteins (ULBP). The genes for ULBPs reside in a cluster of ten related genes, six of which encode potentially functional glycoproteins. ULBP-2 has also been described under the names RaeT1H (retinoic acid early transcript), NKG2DL2, and ALCAN-alpha. ULBP-5 also known as RaeT1G and ULBP-6 also known as RaeT1L. These proteins are distantly related to MHC class I proteins, but they possess only the alpha 1 and alpha 2 Ig-like domains, and they have no capacity to bind peptide or interact with
beta 2‑microglobulin. Some family members, including ULBP-2, are anchored to the membrane via a GPI-linkage, whereas others have transmembrane domains. Engagement of NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. The ULBPs are expressed on some tumor cells and have been implicated in tumor surveillance. Over aa 26-217, ULBP-2 shares 92% and 95% aa sequence identity with the human ULBP-5 and ULBP-6, respectively.

  1. Cosman, D. et al. (2001) Immunity 14:123.
  2. Kubin, M. et al. (2001) Eur. J. Immunol. 31:1428.
  3. Sutherland, C. et al. (2002) J. Immunol. 168:671.
  4. Steinle, A. et al. (2001) Immunogenetics 53:279.
  5. Sutherland, C. et al. (2001) Immunol. Rev. 181:185.
  6. Pende, D. et al. (2002) Cancer Res. 62:6178.
  7. Radosavljevic, M. et al. (2002) Genomics 79:114.
  8. NKG2D and its Ligands (2002) www.RnDSystems.com.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ULBP-2/5/6 Antibody (AF1298)(9)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: Flow Cytometry, ICC, IHC-P, Western Blot.


Filter By Application
Flow Cytometry
(1)
ICC
(1)
IHC-P
(2)
Western Blot
(5)
All Applications
Filter By Species
Human
(9)
All Species
Showing Publications 1 - 9 of 9.
Publications using AF1298 Applications Species
R Okita, A Maeda, K Shimizu, Y Nojima, S Saisho, M Nakata Clinicopathological relevance of tumor expression of NK group 2 member D ligands in resected non-small cell lung cancer Oncotarget, 2019;10(63):6805-6815. 2019 [PMID: 31827723] (IHC-P, Human) IHC-P Human
Natural Killer Cell Evasion Is Essential for Infection by Rhesus Cytomegalovirus PLoS Pathog, 2016;12(8):e1005868. 2016 [PMID: 27580123] (Flow Cytometry, Human) Flow Cytometry Human
Leung W, Vong Q, Lin W, Bouck D, Wendt S, Sullivan E, Li Y, Bari R, Chen T, Leung W PRL-3 mediates the protein maturation of ULBP2 by regulating the tyrosine phosphorylation of HSP60. J Immunol, 2015;194(6):2930-41. 2015 [PMID: 25687758] (Western Blot, Human) Western Blot Human
Cho, Hanbyoul, Chung, Joon-Yon, Kim, Sunghoon, Braunschweig, Till, Kang, Tae Heun, Kim, Jennie, Chung, Eun Joo, Hewitt, Stephen, Kim, Jae-Hoon MICA/B and ULBP1 NKG2D ligands are independent predictors of good prognosis in cervical cancer. BMC Cancer, 2014;14(0):957. 2014 [PMID: 25510288] (IHC-P, Human) IHC-P Human
Heinemann A, Zhao F, Pechlivanis S, Eberle J, Steinle A, Diederichs S, Schadendorf D, Paschen A Tumor suppressive microRNAs miR-34a/c control cancer cell expression of ULBP2, a stress-induced ligand of the natural killer cell receptor NKG2D. Cancer Res., 2012;72(2):460-71. 2012 [PMID: 22102694] (Western Blot, Human) Western Blot Human
Chang YT, Wu CC, Shyr YM, Chen TC, Hwang TL, Yeh TS, Chang KP, Liu HP, Liu YL, Tsai MH, Chang YS, Yu JS Secretome-Based Identification of ULBP2 as a Novel Serum Marker for Pancreatic Cancer Detection. PLoS ONE, 2011;6(5):e20029. 2011 [PMID: 21625447] (Western Blot, Human) Western Blot Human
Fernandez-Messina L, Ashiru O, Aguera-Gonzalez S, Reyburn HT, Vales-Gomez M The human NKG2D ligand ULBP2 can be expressed at the cell surface with or without a GPI anchor and both forms can activate NK cells. J. Cell. Sci., 2011;124(0):321-7. 2011 [PMID: 21224393] (ICC, Human) ICC Human
Ohashi M, Eagle RA, Trowsdale J Post-translational modification of the NKG2D ligand RAET1G leads to cell surface expression of a glycosylphosphatidylinositol-linked isoform. J. Biol. Chem., 2010;285(22):16408-15. 2010 [PMID: 20304922] (Western Blot, Human) Western Blot Human
Schwinn N, Vokhminova D, Sucker A, Textor S, Striegel S, Moll I, Nausch N, Tuettenberg J, Steinle A, Cerwenka A, Schadendorf D, Paschen A Interferon-gamma down-regulates NKG2D ligand expression and impairs the NKG2D-mediated cytolysis of MHC class I-deficient melanoma by natural killer cells. Int. J. Cancer, 2009;124(7):1594-604. 2009 [PMID: 19089914] (Western Blot, Human) Western Blot Human

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