ULBP-2/5/6 Antibody (165903) [PerCP (Peridinin-chlorophyll Protein Complex)]

HT1080 human fibrosarcoma cell line was stained with Mouse Anti-Human ULBP-2/5/6 PerCP-conjugated Monoclonal Antibody (Catalog # FAB1298C, filled histogram) or isotype control antibody (Catalog # IC003C, open histogram). View our protocol for Staining Membrane-associated Proteins.

Product Discontinued

ULBP-2/5/6 Antibody (165903) [PerCP (Peridinin-chlorophyll Protein Complex)] Summary

• Immunogen: BaF3 mouse pro-B cell line transfected with human ULBP-2
  Accession # Q9BZM5
• Specificity: Detects human ULBP-2 and human RAET1L/ULBP-6 in direct ELISA and stains cells transfected with human ULBP-2, human ULBP-5 or human RAET1L/ULBP-6 in flow cytometry. In direct ELISA, 11% cross-reactivity with recombinant human ULBP5 is observed. It does not stain cells transfected with human ULBP-1 or human ULBP-3 in flow cytometry.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ULBP-2/5/6 Antibody (165903) [PerCP (Peridinin-chlorophyll Protein Complex)]

• ALCAN-alpha
• N2DL2
• NKG2DL2
• RAET1H
• RAET1L
• UL16 binding protein 2
• ULBP2
• ULBP-2/5/6

Background

ULBPs activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. Binding of ULBPs ligands to NKG2D induces calcium mobilization and activation of the JAK2, STAT5, ERK and PI3K kinase/Akt signal transduction pathway. The name ULBP derives from the original identification of three proteins, ULBP-1, -2, and -3, as ligands for the human cytomegalovirus glycoprotein UL16; they were designated UL16 binding proteins (ULBP). The genes for ULBPs reside in a cluster of ten related genes, six of which encode potentially functional glycoproteins. ULBP-2 has also been described under the names RaeT1H (retinoic acid early transcript), NKG2DL2, and ALCAN-alpha. ULBP-5 also known as RaeT1G and ULBP-6 also known as RaeT1L. These proteins are distantly related to MHC class I proteins, but they possess only the alpha 1 and alpha 2 Ig-like domains, and they have no capacity to bind peptide or interact with beta 2-microglobulin. Some family members, including ULBP-2, are anchored to the membrane via a GPI-linkage, whereas others have transmembrane domains. Engagement of NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. The ULBPs are expressed on some tumor cells and have been implicated in tumor surveillance. Over aa 26-217, ULBP-2 shares 92% and 95% aa sequence identity with the human ULBP-5 and ULBP-6, respectively.

1. Cosman, D. et al. (2001) Immunity 14:123.
2. Kubin, M. et al. (2001) Eur. J. Immunol. 31:1428.
3. Sutherland, C. et al. (2002) J. Immunol. 168:671.
4. Steinle, A. et al. (2001) Immunogenetics 53:279.
5. Sutherland, C. et al. (2001) Immunol. Rev. 181:185.
6. Pende, D. et al. (2002) Cancer Res. 62:6178.
7. Radosavljevic, M. et al. (2002) Genomics 79:114.
8. NKG2D and its Ligands (2002) www.RnDSystems.com.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ULBP-2/5/6 Antibody (FAB1298C)(2)

Publications using FAB1298C

• SS Kim, C Doherty, M Moghe, A Rait, KF Pirollo, JB Harford, EH Chang Nanomedicine-Based Gene Delivery for a Truncated Tumor Suppressor RB94 Promotes Lung Cancer Immunity Cancers, 2022-10-18;14(20):. 2022-10-18 [PMID: 36291878] (Flow Cytometry, Xenograft)
• I Truxova, L Kasikova, C Salek, M Hensler, D Lysak, P Holicek, P Bilkova, M Holubova, X Chen, R Mikyskova, M Reinis, M Kovar, B Tomalova, JP Kline, L Galluzzi, R Spisek, J Fucikova Calreticulin exposure on malignant blasts correlates with improved natural killer cell-mediated cytotoxicity in acute myeloid leukemia patients Haematologica, 2019-10-03;0(0):. 2019-10-03 [PMID: 31582537] (Flow Cytometry, Human)

Review for ULBP-2/5/6 Antibody (FAB1298C) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer Flow Human 01/21/2015

Summary

• Application: Flow Cytometry
• Sample Tested: See PMID: 20532954
• Species: Human

Bioinformatics

• Uniprot:
  • Q9BZM5()