Biological Strategies: Western Blot: UHRF1 Antibody [NB100-74597] - Detection of Human NP95/UHRF1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis ...read more
Immunohistochemistry: UHRF1 Antibody [NB100-74597] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti- NP95/UHRF1 used at a dilution of 1:200 (1ug/ml). Detection: DAB
Independent Antibodies: Western Blot: UHRF1 Antibody [NB100-74597] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-74597 used for WB at 0.04 mcg/ml (A) and 1 ...read more
Immunohistochemistry-Paraffin: UHRF1 Antibody [NB100-74597] - FFPE section of human basal cell carcinoma. Antibody used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit.
Immunohistochemistry-Paraffin: UHRF1 Antibody [NB100-74597] - FFPE section of human breast carcinoma. Antibody used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit.
Immunoprecipitation: UHRF1 Antibody [NB100-74597] - Detection of human NP95/UHRF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells ...read more
The immunogen recognized by this antibody maps to a region between residue 275 and 325 of human nuclear zinc finger protein Np95 (ubiquitin-like, containing PHD and RING finger domains, 1 ) using the numbering given in entry NP_001041666.1 (GeneID 29128).
Immunogen affinity purified
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Ubiquitin-like PHD and RING finger domain-containing protein 1
ubiquitin-like with PHD and ring finger domains 1
ubiquitin-like, containing PHD and RING finger domains, 1
Ubiquitin-like-containing PHD and RING finger domains protein 1
This gene encodes a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase II alpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint. Multiple transcript variants encoding different isoforms have been found for this gene.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.