UHRF1 Antibody

Western Blot: UHRF1 Antibody [NB100-74597] - Detection of Human NP95/UHRF1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NP95/UHRF1 antibody NB100-74597 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunohistochemistry: UHRF1 Antibody [NB100-74597] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti- NP95/UHRF1 used at a dilution of 1:200 (1ug/ml). Detection: DAB

Western Blot: UHRF1 Antibody [NB100-74597] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-74597 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate.

Immunohistochemistry-Paraffin: UHRF1 Antibody [NB100-74597] - FFPE section of human basal cell carcinoma. Antibody used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit.

Immunohistochemistry-Paraffin: UHRF1 Antibody [NB100-74597] - FFPE section of human breast carcinoma. Antibody used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit.

Immunoprecipitation: UHRF1 Antibody [NB100-74597] - Detection of human NP95/UHRF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NP95/UHRF1 antibody NB100-74597 (lot NB100-74597-3) used for IP at 6 ug per reaction. NP95/UHRF1 was also immunoprecipitated by a previous lot of this antibody (lot NB100-74597-2). For blotting immunoprecipitated NP95/UHRF1, NB100-74597 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

UHRF1 Antibody Summary

• Description: Novus Biologicals Rabbit UHRF1 Antibody (NB100-74597) is a polyclonal antibody validated for use in IHC, WB and IP. Anti-UHRF1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 275 and 325 of human nuclear zinc finger protein Np95 (ubiquitin-like, containing PHD and RING finger domains, 1 ) using the numbering given in entry NP_001041666.1 (GeneID 29128).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: UHRF1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Paraffin 1:250
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Control:

  UHRF1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for UHRF1 Antibody

• E3 ubiquitin-protein ligase UHRF1
• EC 6.3.2
• EC 6.3.2.-
• FLJ21925
• hNp95
• hUHRF1
• HuNp95
• ICBP90
• ICBP90NP95
• Inverted CCAAT box-binding protein of 90 kDa
• NP95
• Nuclear protein 95
• Nuclear zinc finger protein Np95
• RING finger protein 106
• RNF106
• RNF106MGC138707
• Transcription factor ICBP90
• Ubiquitin-like PHD and RING finger domain-containing protein 1
• ubiquitin-like with PHD and ring finger domains 1
• ubiquitin-like, containing PHD and RING finger domains, 1
• Ubiquitin-like-containing PHD and RING finger domains protein 1
• UHRF1

Background

This gene encodes a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase II alpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint. Multiple transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for UHRF1 Antibody (NB100-74597)(2)

Publications using NB100-74597

• Yang C, Wang Y, Zhang F et al. Inhibiting UHRF1 expression enhances radiosensitivity in human esophageal squamous cell carcinoma. Mol Biol Rep. 2013-09-01 [PMID: 23943380] (WB, IHC-P, Human)
• Ma H, Chen H, Guo X et al. M phase phosphorylation of the epigenetic regulator UHRF1 regulates its physical association with the deubiquitylase USP7 and stability. Proc Natl Acad Sci U S A 2012-03-01 [PMID: 22411829]

Bioinformatics

• Gene Symbol: UHRF1
• Entrez:
  • 29128(Human)
• Uniprot:
  • Q96T88(Human)