UHRF1 Antibody

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Biological Strategies: Western Blot: UHRF1 Antibody [NB100-74597] - Detection of Human NP95/UHRF1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis ...read more
Immunohistochemistry: UHRF1 Antibody [NB100-74597] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti- NP95/UHRF1 used at a dilution of 1:200 (1ug/ml). Detection: DAB
Independent Antibodies: Western Blot: UHRF1 Antibody [NB100-74597] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-74597 used for WB at 0.04 mcg/ml (A) and 1 ...read more
Immunohistochemistry-Paraffin: UHRF1 Antibody [NB100-74597] - FFPE section of human basal cell carcinoma. Antibody used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit.
Immunohistochemistry-Paraffin: UHRF1 Antibody [NB100-74597] - FFPE section of human breast carcinoma. Antibody used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit.
Immunoprecipitation: UHRF1 Antibody [NB100-74597] - Detection of human NP95/UHRF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

UHRF1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 275 and 325 of human nuclear zinc finger protein Np95 (ubiquitin-like, containing PHD and RING finger domains, 1 ) using the numbering given in entry NP_001041666.1 (GeneID 29128).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
UHRF1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Paraffin 1:250
  • Immunoprecipitation 2-5 ug/mg lysate
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Control
UHRF1 Overexpression Lysate
Publications
Read Publications using
NB100-74597 in the following applications:

  • 1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for UHRF1 Antibody

  • E3 ubiquitin-protein ligase UHRF1
  • EC 6.3.2
  • EC 6.3.2.-
  • FLJ21925
  • hNP95
  • huNp95
  • ICBP90NP95
  • Inverted CCAAT box-binding protein of 90 kDa
  • Np95
  • Nuclear protein 95
  • Nuclear zinc finger protein Np95
  • RING finger protein 106
  • RNF106MGC138707
  • Transcription factor ICBP90
  • Ubiquitin-like PHD and RING finger domain-containing protein 1
  • ubiquitin-like with PHD and ring finger domains 1
  • ubiquitin-like, containing PHD and RING finger domains, 1
  • Ubiquitin-like-containing PHD and RING finger domains protein 1

Background

This gene encodes a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase II alpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint. Multiple transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for UHRF1 Antibody (NB100-74597)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IHC-P, WB.


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(1)
WB
(1)
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Product General Protocols

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Video Protocols

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Control Lysate(s)

Secondary Antibodies

 

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Blogs on UHRF1.

Chromatin reader domains of DNMT-targeting protein, UHRF1, are responsible for cancerous DNA hypermethylation
By Jamshed Arslan, Pharm. D., PhD. DNA methylation represses transcription of many genes, including tumor suppressor genes. A protein called UHRF1 recruits DNA methyltransferases (DNMTs) to establish and maintain DNA...  Read full blog post.

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Bioinformatics

Gene Symbol UHRF1
Entrez
Uniprot