Recombinant Mouse UDP glucose dehydrogenase His Protein

SDS-Page: Recombinant Mouse UDP glucose dehydrogenase Protein [NBP2-61452] - 15% SDS-Page (3ug)

• Reactivity: Mu
• Applications: PAGE, Enzyme Activity
• Concentration: 0.5 mg/ml

Recombinant Mouse UDP glucose dehydrogenase His Protein Summary

• Description: A recombinant protein with a N-terminalHis-tag and corresponding to the amino acids 1-493 of Mouse UDP glucose dehydrogenase.
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMVEIKKI CCIGAGYVGG PTCSVIAHMC PEIRVTVVDV NEARINAWNS PTLPIYEPGL KEVVESCRGK NLFFSTNIDD AIREADLVFI SVNTPTKTYG MGKGRAADLK YIEACARRIV QNSNGYKIVT EKSTVPVRAA ESIRRIFDAN TKPNLNLQVL SNPEFLAEGT AIKDLKNPDR VLIGGDETPE GQKAVRALCA VYEHWVPKEK ILTTNTWSSE LSKLAANAFL AQRISSINSI SALCEATGAD VEEVATAIGM DQRIGNKFLK ASVGFGGSCF QKDVLNLVYL CEALNLPEVA RYWQQVIDMN DYQRRRFASR IIDSLFNTVT DKKIAILGFA FKKDTGDTRE SSSIYISKYL MDEGAHLHIY DPKVPREQIV VDLSHPGVSA DDQVSRLVTI SKDPYEACDG AHALVICTEW DMFKELDYER IHKKMLKPAF IFDGRRVLDG LHSELQTIGF QIETIGKKVS SKRIPYTPGE IPKFSLQDPP NKKPKV

• Details of Functionality: Specific activity is > 2,500 pmol/min/ug, and is defined as the amount of enzyme that convert 1.0pmole of UDP-glucose to UDP-glucuronate per minute at pH 8.7 at 37C
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: UGDH
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Enzyme Activity
  • SDS-Page
• Theoretical MW: 57.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM MES buffer (pH5.0), 20% glycerol, 150 mM NaCl, 1 mM EDTA
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Mouse UDP glucose dehydrogenase His Protein

• EC 1.1.1.22
• GDH
• UDPGDHUDP-glucose dehydrogenase
• UDP-Glc dehydrogenase
• UDP-GlcDH
• UDP-glucose 6-dehydrogenase
• UGD
• uridine diphospho-glucose dehydrogenase

Background

UDP glucose dehydrogenase is encoded by this gene converts UDP-glucose to UDP-glucuronate and thereby participates in the biosynthesis of glycosaminoglycans such as hyaluronan, chondroitin sulfate, and heparan sulfate. These glycosylated compounds are common components of the extracellular matrix and likely play roles in signal transduction, cell migration, and cancer growth and metastasis. The expression of this gene is up-regulated by transforming growth factor beta and down-regulated by hypoxia. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: UGDH