Recombinant Human UDP glucose dehydrogenase Protein

Images

 
SDS-Page: Recombinant Human UDP glucose dehydrogenase Protein [NBP1-45276] - 15% SDS-PAGE (3ug)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Func, PAGE
Concentration
0.5 mg/ml

Order Details

Recombinant Human UDP glucose dehydrogenase Protein Summary

Description
A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-494 of Human UDP glucose dehydrogenase

Source: E.coli

Amino Acid Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSELEM FEIKKICCIG AGYVGGPTCS VIAHMCPEIR VTVVDVNESR INAWNSPTLP IYEPGLKEVV ESCRGKNLFF STNIDDAIKE ADLVFISVNT PTKTYGMGKG RAADLKYIEA CARRIVQNSN GYKIVTEKST VPVRAAESIR RIFDANTKPN LNLQVLSNPE FLAEGTAIKD LKNPDRVLIG GDETPEGQRA VQALCAVYEH WVPREKILTT NTWSSELSKL AANAFLAQRI SSINSISALC EATGADVEEV ATAIGMDQRI GNKFLKASVG FGGSCFQKDV LNLVYLCEAL NLPEVARYWQ QVIDMNDYQR RRFASRIIDS LFNTVTDKKI AILGFAFKKD TGDTRESSSI YISKYLMDEG AHLHIYDPKV PREQIVVDLS HPGVSEDDQV SRLVTISKDP YEACDGAHAV VICTEWDMFK ELDYERIHKK MLKPAFIFDG RRVLDGLHNE LQTIGFQIET IGKKVSSKRI PYAPSGEIPK FSLQDPPNKK PKV

Details of Functionality
Specific activity is > 2500 pmol/min/ug, and is defined as the amount of enzyme that convert 1.0pmole of UDP-glucose to UDP-glucuronate per minute at pH 8.7 at 37C.
Source
E. coli
Protein/Peptide Type
Biologically Active Protein
Gene
UGDH
Purity
>95% pure by SDS-PAGE

Applications/Dilutions

Theoretical MW
59.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer(pH 8.0) containing 20% glycerol, 1mM DTT, 0.1M NaCl, and 1mM EDTA.
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
>95% pure by SDS-PAGE

Alternate Names for Recombinant Human UDP glucose dehydrogenase Protein

  • EC 1.1.1.22
  • GDH
  • UDPGDHUDP-glucose dehydrogenase
  • UDP-Glc dehydrogenase
  • UDP-GlcDH
  • UDP-glucose 6-dehydrogenase
  • UGD
  • uridine diphospho-glucose dehydrogenase

Background

UGDH is a member of the UDP-glucose/GDP-mannose dehydrogenase family and is a ubiquitously expressed protein most abundant in the liver. This protein converts UDP-glucose to UDP-glucuronate and thereby participates in the biosynthesis of glycosaminoglycans such as hyaluronan, chondroitin sulfate, and heparan sulfate. These glycosylated compounds are common components of the extracellular matrix and likely play roles in signal transduction, cell migration, and cancer growth and metastasis. Recombinant human UGDH protein, fused to his-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB110-41404
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC
NBP2-02615
Species: Hu, Mk, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NB100-91662
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-02615
Species: Hu, Mk, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP1-32259
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP2-01506
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP2-45978
Species: Hu
Applications: WB, Flow, IHC
NBP1-87069
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP, PAGE
NBP1-81838
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP2-16679
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NB100-65214
Species: Hu, Mu, Rt, Ma
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-31470
Species: Hu, Mu, Rt
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NBP2-52557
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready, IF
NBP2-02164
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP2-29940
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC-P
NBP2-02541
Species: Hu, Pm
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-32398
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP2-34980
Species: Hu
NBP1-54755
Species: Hu
Applications: WB

Publications for UDP glucose dehydrogenase Biologically Active Protein (NBP1-45276) (0)

There are no publications for UDP glucose dehydrogenase Biologically Active Protein (NBP1-45276).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for UDP glucose dehydrogenase Biologically Active Protein (NBP1-45276) (0)

There are no reviews for UDP glucose dehydrogenase Biologically Active Protein (NBP1-45276). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

View specific protocols for UDP glucose dehydrogenase Biologically Active Protein (NBP1-45276): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for UDP glucose dehydrogenase Biologically Active Protein (NBP1-45276) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional UDP glucose dehydrogenase Products

Bioinformatics Tool for UDP glucose dehydrogenase Biologically Active Protein (NBP1-45276)

Discover related pathways, diseases and genes to UDP glucose dehydrogenase Biologically Active Protein (NBP1-45276). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for UDP glucose dehydrogenase Biologically Active Protein (NBP1-45276)

Discover more about diseases related to UDP glucose dehydrogenase Biologically Active Protein (NBP1-45276).
 

Pathways for UDP glucose dehydrogenase Biologically Active Protein (NBP1-45276)

View related products by pathway.

PTMs for UDP glucose dehydrogenase Biologically Active Protein (NBP1-45276)

Learn more about PTMs related to UDP glucose dehydrogenase Biologically Active Protein (NBP1-45276).
 

Research Areas for UDP glucose dehydrogenase Biologically Active Protein (NBP1-45276)

Find related products by research area.

Blogs on UDP glucose dehydrogenase

There are no specific blogs for UDP glucose dehydrogenase, but you can read our latest blog posts.

Customers Who Bought This Also Bought

SDHB Antibody
NBP1-87069

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human UDP glucose dehydrogenase Protein and receive a gift card or discount.

Bioinformatics

Gene Symbol UGDH