UBPY/USP8 Antibody

Western Blot: UBPY/USP8 Antibody [NBP1-50001] - Detection of Human USP8 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-USP8 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. USP8 was also immunoprecipitated by rabbit anti-USP8 antibody which recognizes a downstream epitope. For blotting immunoprecipitated USP8, the ReliaBLOT(R) Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).

Immunohistochemistry-Paraffin: UBPY/USP8 Antibody [NBP1-50001] - FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-USP8 (NBP1-50001) used at a dilution of 1:200 (1ug/ml). Detection: DAB

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

UBPY/USP8 Antibody Summary

• Immunogen: The immunogen for this product maps to a region between residue 400 and 450 of human Ubiquitin Specific Peptidase 8 using the numbering given in entry NP_005145.3 (GeneID 9101).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: USP8
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for UBPY/USP8 Antibody

• Deubiquitinating enzyme 8
• EC 3.1.2.15
• EC 3.4.19.12
• HumORF8
• KIAA0055hUBPy
• MGC129718
• ubiquitin carboxyl-terminal hydrolase 8
• Ubiquitin isopeptidase Y
• ubiquitin specific peptidase 8
• ubiquitin specific protease 8
• ubiquitin thioesterase 8
• Ubiquitin thiolesterase 8
• Ubiquitin-specific-processing protease 8
• UBPY
• UBPYFLJ34456
• USP8

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: USP8
• Entrez:
  • 9101(Human)
• Uniprot:
  • P40818(Human)