Ubiquitin B Antibody (JM09-67)

Western Blot: Ubiquitin B Antibody (JM09-67) [NBP2-66759] - Analysis of K63-linkage Specific Ubiquitin on Hela cells lysates using anti-K63-linkage Specific Ubiquitin antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: Ubiquitin B Antibody (JM09-67) [NBP2-66759] - Staining K63-linkage Specific Ubiquitin in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Ubiquitin B Antibody (JM09-67) [NBP2-66759] - Analysis of paraffin-embedded human spleen tissue using anti-K63-linkage Specific Ubiquitin antibody. Counter stained with hematoxylin.

Immunocytochemistry/Immunofluorescence: Ubiquitin B Antibody (JM09-67) [NBP2-66759] - Staining K63-linkage Specific Ubiquitin in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Ubiquitin B Antibody (JM09-67) [NBP2-66759] - Staining K63-linkage Specific Ubiquitin in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Ubiquitin B Antibody (JM09-67) [NBP2-66759] - Analysis of paraffin-embedded human colon cancer tissue using anti-K63-linkage Specific Ubiquitin antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Ubiquitin B Antibody (JM09-67) [NBP2-66759] - Analysis of paraffin-embedded human liver cancer tissue using anti-K63-linkage Specific Ubiquitin antibody. Counter stained with hematoxylin.

Product Discontinued

Ubiquitin B Antibody (JM09-67) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within human k63-linkage specific ubiquitin aa 36-85. (SwissProt: P0CG47 Human; SwissProt: P0CG48 Human; SwissProt: P62979 Human; SwissProt: P62987 Human; SwissProt: P0CG49 Mouse; SwissProt: P62991 Mouse; SwissProt: P0CG51 Rat; SwissProt: P62989 Rat)
• Localization: Cytoplasm, Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: UBB
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Ubiquitin B Antibody (JM09-67)

• FLJ25987
• MGC8385
• polyubiquitin B
• polyubiquitin-B
• RPS27A
• UBA52
• UBC
• ubiquitin B
• Ubiquitin+1

Background

ubiquitin B encodes ubiquitin, one of the most conserved proteins known. Ubiquitin is required for ATP-dependent, nonlysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimer's and Down syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: UBB