Ubiquitin [p Ser65] Antibody (1272B) [Unconjugated]

Western blot shows lysates of Di-Ubiquitin (negative control), Phospho-Tetra-Ubiquitin (S65), Phospho-Ubiquitin (S65), K48 linked Phosopho-Tetra-Ubiquitin (S65), and K48 linked Ubiquitin 2-7 (negative control). PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human Phospho-Ubiquitin (S65) Monoclonal Antibody (Catalog # MAB9866, left panel) or 0.1 µg/mL of Rabbit Anti-Human Ubiquitin Monoclonal Antibody (Catalog # MAB8595, right panel), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-Ubiquitin (S65) at approximately 10, 30, and 35 kDa (as indicated on the left panel). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Product Discontinued

Ubiquitin [p Ser65] Antibody (1272B) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide containing human Phospho-Ubiquitin (S65)
  Accession # P0CG47
• Modification: p Ser65
• Specificity: Detects Ubiquitin when phosphorylated at Serine 65.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: UBB
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Ubiquitin [p Ser65] Antibody (1272B) [Unconjugated]

• RPS27A
• UBA52
• UBB ubiquitin B
• UBB
• UBC
• Ubiquitin

Background

Ubiquitin is a 76 amino acid, highly conserved nuclear and cytoplasmic protein. It is found exclusively in eukaryotes, becomes covalently attached to substrate proteins by enzymes in the Ubiquitin-Proteosome Pathway (UPP) and has a major role in targeting cellular proteins for the ATP-dependent degradation by the 26S proteosome. Ubiquitination also affects proteosome-independent events such as protein localization, activity and function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: UBB
• Uniprot:
  • P0CG47()