Ubiquilin 2 Antibody (5F5)

Images

 
Western Blot: Ubiquilin 2 Antibody (5F5) [H00029978-M03] - UBQLN2 shRNA knockdown in SH-SY5Y cells. First lane is the ladder, then treatment with scrambled shRNA and third lane is UBQLN2 knockdown. 30 ug of the protein ...read more
Immunocytochemistry/ Immunofluorescence: Ubiquilin 2 Antibody (5F5) [H00029978-M03] - Analysis of monoclonal antibody to UBQLN2 on A-431 cell. Antibody concentration 10 ug/ml
Western Blot: Ubiquilin 2 Antibody (5F5) [H00029978-M03] - Analysis of UBQLN2 expression in PC-12 (Cat # L012V1).
Western Blot: Ubiquilin 2 Antibody (5F5) [H00029978-M03] - Analysis of UBQLN2 expression in Raw 264.7 (Cat # L024V1).
Western Blot: Ubiquilin 2 Antibody (5F5) [H00029978-M03] - Analysis of UBQLN2 expression in A-431 (Cat # L015V1).
Western Blot: Ubiquilin 2 Antibody (5F5) [H00029978-M03] - Analysis of UBQLN2 expression in NIH/3T3 (Cat # L018V1).
ELISA: Ubiquilin 2 Antibody (5F5) [H00029978-M03] - Detection limit for recombinant GST tagged UBQLN2 is approximately 0.03ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
5F5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Ubiquilin 2 Antibody (5F5) Summary

Immunogen
UBQLN2 (NP_038472, 555 a.a. ~ 624 a.a) partial recombinant protein with GST tag.PNQQFIQQMVQALAGANAPQLPNPEVRFQQQLEQLNAMGFLNREANLQALIATGGDINAAIERLLGSQPS
Specificity
UBQLN2 (5F5)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
UBQLN2
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA. Immunohistochemistry was reported in scientific literature. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 24475300)
Reviewed Applications
Read 1 Review rated 3
using
H00029978-M03 in the following applications:

Publications
Read Publications using
H00029978-M03 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Ubiquilin 2 Antibody (5F5)

  • Chap1
  • CHAP1/DSK2
  • DSK2 homolog
  • DSK2
  • FLJ10167
  • FLJ56541
  • hPLIC-2
  • N4BP4LIC-2
  • Nedd4 binding protein 4
  • PLIC-2
  • PLIC2Dsk2
  • Protein linking IAP with cytoskeleton 2
  • RIHFB2157
  • ubiquilin 2
  • ubiquilin-2
  • Ubiquitin-like product Chap1/Dsk2

Background

This gene encodes an ubiquitin-like protein (ubiquilin) that shares high degree of similarity with related products in yeast, rat and frog. Ubiquilins contain a N-terminal ubiquitin-like domain and a C-terminal ubiquitin-associated domain. They physically associate with both proteasomes and ubiquitin ligases, and thus thought to functionally link the ubiquitination machinery to the proteasome to affect in vivo protein degradation. This ubiquilin has also been shown to bind the ATPase domain of the Hsp70-like Stch protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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H00029978-M03
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for Ubiquilin 2 Antibody (H00029978-M03)(22)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
ICC/IF
(1)
IHC
(1)
IHC-Fr
(1)
IHC-P
(4)
WB
(6)
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Human
(5)
Mouse
(3)
Rat
(3)
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Showing Publications 1 - 10 of 22. Show All 22 Publications.
Publications using H00029978-M03 Applications Species
Wang CH, Huang YC, Chen PY et al. USP5/Leon deubiquitinase confines postsynaptic growth by maintaining ubiquitin homeostasis through Ubiquilin. Elife 2017 May 10 [PMID: 28489002]
Riku Y, Watanabe H, Yoshida M et al. Marked Involvement of the Striatal Efferent System in TAR DNA-Binding Protein 43 kDa-Related Frontotemporal Lobar Degeneration and Amyotrophic Lateral Sclerosis. J Neuropathol Exp Neurol 2016 Jun 26 [PMID: 27346748]
Arvaniti M, Jensen MM, Soni N et al. Functional interaction between Lypd6 and nicotinic acetylcholine receptors J. Neurochem. 2016 Jun 25 [PMID: 27344019] (WB, Rat) WB Rat
Wear MP, Kryndushkin D, O'Meally R et al. Proteins with Intrinsically Disordered Domains Are Preferentially Recruited to Polyglutamine Aggregates. PLoS ONE 2015 Aug 31 [PMID: 26317359] (WB, Rat) WB Rat
Shimada K, Fujii T, Tatsumi Y et al. Ubiquilin2 as a novel marker for detection of urothelial carcinoma cells in urine. Diagn Cytopathol 2016 Jan [PMID: 26303000]
Zeng L, Wang B, Merillat SA et al. Differential recruitment of UBQLN2 to nuclear inclusions in the polyglutamine diseases HD and SCA3 Neurobiol. Dis. 2015 Jun 30 [PMID: 26141599] (WB, IHC-P, Mouse, Human) WB, IHC-P Mouse, Human
Porta S, Kwong LK, Trojanowski JQ, Lee VM. Drosha Inclusions Are New Components of Dipeptide-Repeat Protein Aggregates in FTLD-TDP and ALS C9orf72 Expansion Cases. J Neuropathol Exp Neurol. 2015 Apr 01 [PMID: 25756586]
Wu Q, Liu M, Huang C et al. Pathogenic Ubqln2 gains toxic properties to induce neuron death. Acta neuropathol. 2014 Nov 12 [PMID: 25388785] (WB, Rat) WB Rat
Fawzi Aa, Simonett Jm, Purta P et al. Clinicopathologic report of ocular involvement in ALS patients with C9orf72 mutation. Amyotroph Lateral Scler Frontotemporal Degener. 2014 Oct 16 [PMID: 25319030] (IHC-P, Human) IHC-P Human
Konno T, Tada M, Shiga A et al. C9ORF72 repeat-associated non-ATG-translated polypeptides are distributed independently of TDP-43 in a Japanese patient with c9ALS. Neuropathol Appl Neurobiol. 2014 May 21 [PMID: 24861677]
Show All 22 Publications.

Review for Ubiquilin 2 Antibody (H00029978-M03) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using H00029978-M03:
Filter by Applications
WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot Ubiquilin 2 H00029978-M03
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reviewed by:
Anonymous
WB Human 02/24/2017
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Summary

ApplicationWestern Blot
Sample TestedSH-SY5Y cell lysate
SpeciesHuman
LotGA061-5F5

Comments

CommentsThe samples were lysed in Ripa buffer, followed by sonication. 30 ug of the protein sample was loaded on the gel and transferred to the nitrocellulose membrane. Blocked for 45 min at RT in 5 % milk in TBST, overnight incubation in primary antibodies at 4 °C in 5 % milk in TBST, washed with TBST 3x for 10 min, incubated in secondary antibodies (Goat Anti-mouse DyLight 550) at RT for 2 h and then imaged using BioRad ChemiDoc. Images were quantified using ImageLab Software (BioRad).
Knockdown of the upper band with shRNA for UBQLN2 was about 82 %, and of the lower band only about 8 %, suggesting a high specificity of the used shRNA, yet if comparing to the data provided in the antibody datasheet, my shRNA wouldn’t really be targeting UBQLN2, but some undefined band. It is possible that the antibody is not able to differentiate between UBQLN2 and the member of the same family, UBQLN1. UBQLN1 is slightly smaller than UBQLN2, but they both share a significant similarity at the C-terminus, from which the immunogen for the UBQLN2 antibody was raised.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Ubiquilin 2 Antibody (H00029978-M03). (Showing 1 - 1 of 1 FAQs).

  1. Do you know if the mAb detects ubiquilin 1?
    • Based on the sequence homology, it looks like there could be some cross-reactivity with Ubiquilin 1. The immunogen sequence has 92% homology with bovine Ubiquilin 1.

Secondary Antibodies

 

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Recent Reviews

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Anonymous
02/24/2017
Application: WB
Species: Human

Bioinformatics

Gene Symbol UBQLN2
Entrez
OMIM
Uniprot