Western Blot: Ubiquilin 2 Antibody (5F5) [H00029978-M03] - UBQLN2 shRNA knockdown in SH-SY5Y cells. First lane is the ladder, then treatment with scrambled shRNA and third lane is UBQLN2 knockdown. 30 ug of the protein ...read more
Immunocytochemistry/ Immunofluorescence: Ubiquilin 2 Antibody (5F5) [H00029978-M03] - Analysis of monoclonal antibody to UBQLN2 on A-431 cell. Antibody concentration 10 ug/ml
Western Blot: Ubiquilin 2 Antibody (5F5) [H00029978-M03] - Analysis of UBQLN2 expression in PC-12 (Cat # L012V1).
Western Blot: Ubiquilin 2 Antibody (5F5) [H00029978-M03] - Analysis of UBQLN2 expression in Raw 264.7 (Cat # L024V1).
Western Blot: Ubiquilin 2 Antibody (5F5) [H00029978-M03] - Analysis of UBQLN2 expression in A-431 (Cat # L015V1).
Western Blot: Ubiquilin 2 Antibody (5F5) [H00029978-M03] - Analysis of UBQLN2 expression in NIH/3T3 (Cat # L018V1).
ELISA: Ubiquilin 2 Antibody (5F5) [H00029978-M03] - Detection limit for recombinant GST tagged UBQLN2 is approximately 0.03ng/ml as a capture antibody.
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA. Immunohistochemistry was reported in scientific literature. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 24475300)
Read 1 Review rated 3 using H00029978-M03 in the following applications:
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
PBS (pH 7.4)
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Ubiquilin 2 Antibody (5F5)
Nedd4 binding protein 4
Protein linking IAP with cytoskeleton 2
Ubiquitin-like product Chap1/Dsk2
This gene encodes an ubiquitin-like protein (ubiquilin) that shares high degree of similarity with related products in yeast, rat and frog. Ubiquilins contain a N-terminal ubiquitin-like domain and a C-terminal ubiquitin-associated domain. They physically associate with both proteasomes and ubiquitin ligases, and thus thought to functionally link the ubiquitination machinery to the proteasome to affect in vivo protein degradation. This ubiquilin has also been shown to bind the ATPase domain of the Hsp70-like Stch protein.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
The samples were lysed in Ripa buffer, followed by sonication. 30 ug of the protein sample was loaded on the gel and transferred to the nitrocellulose membrane. Blocked for 45 min at RT in 5 % milk in TBST, overnight incubation in primary antibodies at 4 °C in 5 % milk in TBST, washed with TBST 3x for 10 min, incubated in secondary antibodies (Goat Anti-mouse DyLight 550) at RT for 2 h and then imaged using BioRad ChemiDoc. Images were quantified using ImageLab Software (BioRad). Knockdown of the upper band with shRNA for UBQLN2 was about 82 %, and of the lower band only about 8 %, suggesting a high specificity of the used shRNA, yet if comparing to the data provided in the antibody datasheet, my shRNA wouldn’t really be targeting UBQLN2, but some undefined band. It is possible that the antibody is not able to differentiate between UBQLN2 and the member of the same family, UBQLN1. UBQLN1 is slightly smaller than UBQLN2, but they both share a significant similarity at the C-terminus, from which the immunogen for the UBQLN2 antibody was raised.
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