![Immunoprecipitation: UBQLN4/CIP75 Antibody [NBP3-14725] - Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from HEK293T cellsprepared using NETN lysis buffer. Antibodies: Rabbit antiUBQLN4 recombinant monoclonal antibodyused for IP at 20 ul/mg lysate. UBQLN4was also immunoprecipitated by others rabbit anti-UBQLN4antibodies. For blottingimmunoprecipitated was used at1:1000. Chemiluminescence with an exposure time of 10seconds.](http://images.novusbio.com/fullsize/UBQLN4-CIP75-Antibody-Immunoprecipitation-NBP3-14725-img0001.jpg "Immunoprecipitation: UBQLN4/CIP75 Antibody [NBP3-14725] - Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from HEK293T cellsprepared using NETN lysis buffer. Antibodies: Rabbit antiUBQLN4 recombinant monoclonal antibodyused for IP at 20 ul/mg lysate. UBQLN4was also immunoprecipitated by others rabbit anti-UBQLN4antibodies. For blottingimmunoprecipitated was used at1:1000. Chemiluminescence with an exposure time of 10seconds.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, IP |
| Clone | BLR145J |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | Novus Biologicals Rabbit UBQLN4/CIP75 Antibody (BLR145J) (NBP3-14725) is a recombinant monoclonal antibody validated for use in IHC, WB, Flow, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Additional Information | Recombinant Monoclonal |
| Immunogen | residues 2-601 (full length) |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | UBQLN4 |
| Purity | >95% |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. |
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| Storage | Store at 2 - 8 C / 1 year from date of receipt |
| Buffer | Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and |
| Preservative | 0.09% Sodium Azide |
| Purity | >95% |
| Publication using NBP3-14725 | Applications | Species |
|---|---|---|
| Xu M, Wei J, Feng X et al. LncDARS‐AS1 Regulates ATP1A1 Stability and Enhances Na + /K + ATPase Activity to Promote Osteosarcoma Metastasis Advanced Science 2025-07-15 [PMID: 40665639] |
Secondary Antibodies |
Isotype Controls |
Research Areas for UBQLN4/CIP75 Antibody (NBP3-14725)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | UBQLN4 |
![Western Blot: UBQLN4/CIP75 Antibody [NBP3-14725] -Whole cell lysate (50 ug) from HEK293T, OVCAR-3, MCF-7, and K-562 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-UBQLN4 recombinant monoclonal antibody [BLR145J] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-COPB2 antibody.](http://images.novusbio.com/fullsize/nbp3-14725_rabbit-ubqln4-cip75-mab-western-blot-1472023122256..jpg "Western Blot: UBQLN4/CIP75 Antibody [NBP3-14725] -Whole cell lysate (50 ug) from HEK293T, OVCAR-3, MCF-7, and K-562 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-UBQLN4 recombinant monoclonal antibody [BLR145J] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-COPB2 antibody.")
![Western Blot- UBQLN4/CIP75 Antibody [NBP3-14725] -Whole cell lysate (50 ug) prepared using NETN buffer from MDA-MB-231 cells (WT and KO). Antibodies: Rabbit antiUBQLN4 recombinant monoclonal antibody [BLR145J] used for WB at 1:1000. Secondary: HRPconjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody.](http://images.novusbio.com/fullsize/nbp3-14725_rabbit-ubqln4-cip75-mab-western-blot-1472023122446..jpg "Western Blot- UBQLN4/CIP75 Antibody [NBP3-14725] -Whole cell lysate (50 ug) prepared using NETN buffer from MDA-MB-231 cells (WT and KO). Antibodies: Rabbit antiUBQLN4 recombinant monoclonal antibody [BLR145J] used for WB at 1:1000. Secondary: HRPconjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody.")
![IHC-P: UBQLN4/CIP75 Antibody [NBP3-14725] -y: Rabbit anti-UBQLN4 recombinant monoclonal antibody [BLR145J]. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.](http://images.novusbio.com/fullsize/nbp3-14725_rabbit-ubqln4-cip75-mab-immunohistochemistry-paraffin-1472023122614..jpg "IHC-P: UBQLN4/CIP75 Antibody [NBP3-14725] -y: Rabbit anti-UBQLN4 recombinant monoclonal antibody [BLR145J]. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.")
![ICC/IF: UBQLN4/CIP75 Antibody [NBP3-14725] -: Rabbit anti-UBQLN4 recombinant monoclonal antibody [BLR145J]. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/nbp3-14725_rabbit-ubqln4-cip75-mab-immunocytochemistry-immunofluorescence-1472023122751..jpg "ICC/IF: UBQLN4/CIP75 Antibody [NBP3-14725] -: Rabbit anti-UBQLN4 recombinant monoclonal antibody [BLR145J]. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.")
![Western Blot Rad23 Antibody [Unconjugated]](https://images.novusbio.com/images2/Rad23_AF4555_Western_Blot_5498.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
