Rad23 Antibody [Unconjugated]

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Raji human Burkitt's lymphoma cell line, MCF-7 human breast cancer cell line, Nb2-11 rat lymphoma cell line, and mouse brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat/Yeast Rad23 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4555) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Rad23 at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu, Rt, Ye
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Rad23 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant yeast Rad23
  Accession # P32628
• Specificity: Detects human, mouse, rat, and yeast Rad23 protein in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: RAD23A
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Rad23 Antibody [Unconjugated]

• hHR23
• hHR23A
• HR23A
• Rad23

Background

Rad23 is an evolutionarily conserved protein that is important for nucleotide excision repair. All eukaryotic Rad23 proteins contain an amino-terminal UbL domain that bears a striking resemblance to ubiquitin. Rad23 has a proteolytic function in DNA repair via its interaction with the 26S proteosome through the UbL domain. The elimination of this domain causes sensitivity to ultraviolet radiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Rad23 Antibody (AF4555)(1)

Publication using AF4555

• Acharya, N;Daniel, E;Dao, TP;Mulvey, E;Kraut, D;Roelofs, J;Castañeda, CA; STI1 domain dynamically engages transient helices in disordered regions to drive self-association and phase separation of yeast ubiquilin Dsk2 bioRxiv : the preprint server for biology 2025-03-14 [PMID: 40161686] (Western Blot, Yeast - Saccharomyces cerevisiae)

Bioinformatics

• Gene Symbol: RAD23A
• Uniprot:
  • P32628()