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Ubiquilin 1 Antibody

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Western Blot: Ubiquilin 1 Antibody [NB120-3341] - Analysis of ubiquilin on CHO cell extract.
Western Blot: Ubiquilin 1 Antibody [NB120-3341] - Analysis of ubiquilin on CHO cell extract.

Product Details

Summary
Reactivity Hu, Mu, ChHa, HaSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

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Ubiquilin 1 Antibody Summary

Immunogen
Synthetic Peptide: A(2) E S A E S G G P P G A Q D S A A(18)
Specificity
Ubiquilin 1
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
UBQLN1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
Application Notes
WB: Detects an approx. 63 kDa protein representing Ubiquilin from CHO cell extract.
Reviewed Applications
Read 1 Review rated 4
using
NB120-3341 in the following application:

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 16945923).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 1 mg/ml BSA
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Ubiquilin 1 Antibody

  • DA41
  • DA41FLJ90054
  • DSK2
  • hPLIC-1
  • PLIC1
  • PLIC-1
  • PLIC-1UBQN
  • Protein linking IAP with cytoskeleton 1
  • Ubiquilin 1
  • ubiquilin-1
  • UBQLN1
  • UBQN
  • XDRP1

Background

Ubiquilin 1 encodes an ubiquitin-like protein (ubiquilin) that shares high degree of similarity with related products in yeast, rat and frog. Ubiquilins contain a N-terminal ubiquitin-like domain and a C-terminal ubiquitin-associated domain. They physically associate with both proteasomes and ubiquitin ligases, and thus are thought to functionally link the ubiquitination machinery to the proteasome to affect in vivo protein degradation. This ubiquilin has also been shown to modulate accumulation of presenilin proteins, and is found in lesions associated with Alzheimer's and Parkinson's disease. Two transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: beta Amyloid Antibody (MOAB-2) [NBP2-13075] - Immunofluorescent detection of beta Amyloid with MOAB-2 in the subiculim of 1-, 2- and 4- month old 5xFAD mice. Scale bar 20 um (from PMID: 22423893).
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Western Blot: Presenilin-1 Antibody (APS 18) [NB100-74510] - Analysis of THP-1 cell lysate.
Immunocytochemistry/Immunofluorescence: Presenilin-1 Antibody (APS 18) [NB100-74510] - Staining of PS1 in mouse fibroblasts.
Immunocytochemistry/Immunofluorescence: Presenilin-1 Antibody (APS 18) [NB100-74510] - Analysis of Presenilin 1 using Presenilin 1 Monoclonal antibody (APS 18) shows staining in MCF-7 cells. Presenilin 1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Presenilin 1 at a dilution of 1:20-1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
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Immunohistochemistry-Paraffin: S5a/Angiocidin Antibody [NBP2-37888] - Staining of human testis shows strong cytoplasmic and nuclear positivity in cells in seminiferous ducts.
Immunocytochemistry/Immunofluorescence: S5a/Angiocidin Antibody [NBP2-37888] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
Immunohistochemistry-Paraffin: S5a/Angiocidin Antibody [NBP2-37888] - Staining of human cerebral cortex, liver, lymph node and testis using Anti-PSMD4 antibody NBP2-37888 (A) shows similar protein distribution across tissues to independent antibody NBP1-90821 (B).
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Western Blot: Ubiquilin 2 Antibody (5F5) [H00029978-M03] - UBQLN2 shRNA knockdown in SH-SY5Y cells. First lane is the ladder, then treatment with scrambled shRNA and third lane is UBQLN2 knockdown. 30 ug of the protein sample was loaded on the gel and transferred to the nitrocellulose membrane. Overnight incubation in primary antibodies at 4 degrees C in 5 % milk in TBST. Then incubated in secondary antibodies (Goat Anti-mouse DyLight 550) at RT for 2 h, and then imaged. Knockdown of the upper band with shRNA for UBQLN2 was about 82 %, and of the lower band only about 8 %, suggesting a high specificity of the used shRNA. This image was submitted via customer Review.
Western Blot: Ubiquilin 2 Antibody (5F5) [H00029978-M03] - Generation of transgenic mice that overexpress human ubiquilin-1. Immunoblots of equal amounts of total brain lysates from 12 month-old WT mouse, 12 month-old Ubqln-1 48 transgenic mouse, 12 month-old Ubqln-1 62 transgenic mouse and end stage 15 week-old R6/2 transgenic mouse. The top panel was probed with a monoclonal anti-ubiquilin antibody (Invitrogen antibody clone 3D5E2) and the lower panel with a different monoclonal anti-Ubiquilin 2 antibody (Novus antibody clone 5F5). Note two immunoreactive ubiquilin bands are seen at 70 kDa and at 90 kDa, which we presume is a modified form of ubiquilin. Both blots were also probed for actin to ensure equal loading. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0087513) licensed under a CC-BY licence.
Western Blot: Ubiquilin 2 Antibody (5F5) [H00029978-M03] - Confirmation of polyQ-associated proteins from PC-12 cells identified by TAPI. Western blot analysis of TAPI-purified polyQ aggregates from PC-12 cells confirms the presence of several disease-associated proteins only in the Htt-Q74 samples. All proteins migrated near their predicted molecular weights. For control, the TAPI procedure was conducted in parallel on the induced Htt-Q23 cell line (FUS, TDP-43, UBQLN2, HNRNPA1) or the un-induced Htt-Q74 cell line (CLINT1, HSPA8, RAD23B, SGTA). Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0136362), licensed under a CC-BY licence.
(1 Review)
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Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, KD, WB
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Immunocytochemistry/Immunofluorescence: Presenilin-2 Antibody (APS 26) [NB100-74513] - Staining of mouse fibroblasts.
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236-EG
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Western Blot: CD47 Antibody (B6H12.2) - Azide Free [NBP2-31106] - Detection of CD47 expression on two hematological cancer cell lines, Jurkat and Raji. WB image submitted by a verified customer review.
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Flow Cytometry: Rad23 Antibody (3E3F4) [NBP2-52549] - Analysis of HeLa cells using RAD23A mouse mAb (green) and negative control (red).
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Flow Cytometry: p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on A549 cells with p53 (PAb240) NB200-103AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
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Western Blot: Proteasome 19S 10B Antibody [NB100-1483] - Staining of HeLa (A), U251 (B), KNRK (C) with antibody at 1 ug/mL and NIH3T3 (D) cell lysate with antibody at 0.3 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/Immunofluorescence: Proteasome 19S 10B Antibody [NB100-1483] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic and some nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Flow Cytometry: Proteasome 19S 10B Antibody [NB100-1483] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Immunohistochemistry-Paraffin: DDI1 Antibody [NBP1-31417] - Paraffin-embedded OVCA, using antibody at 1:100 dilution.
Western Blot: DDI1 Antibody [NBP1-31417] - Sample(30 ug whole cell lysate) A:MOLT4 7.5% SDS PAGE, antibody diluted at 1:1000.
Immunocytochemistry/Immunofluorescence: DDI1 Antibody [NBP1-31417] - Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DDI1 protein stained by DDI1 antibody diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 um.
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Applications: ICC/IF, IHC, IHC-P, WB
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Immunohistochemistry-Paraffin: Utrophin Antibody [NBP1-84074] - Staining of human kidney shows moderate membranous positivity in cells in glomeruli and cells in tubules.
Immunohistochemistry-Paraffin: Utrophin Antibody [NBP1-84074] - Staining of human stomach shows moderate membranous positivity in glandular cells.
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Western Blot: Ribophorin I Antibody (OTI3H3) [NBP2-01294] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Ribophorin I monoclonal antibody.
Immunocytochemistry/Immunofluorescence: Ribophorin I Antibody (OTI3H3) [NBP2-01294] - expression of RPN1 on pca cell lines vs normal. Image from verified customer review.
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(1 Publication)
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Immunohistochemistry-Paraffin: TFF1/pS2 Antibody [NBP1-90813] - Staining of human stomach shows high expression.
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Applications: IHC, IHC-P, WB
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DAN/NBL1 Antibody [Unconjugated]
(1 Publication)
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Applications: Block, WB
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Immunohistochemistry-Paraffin: FUS Antibody [NB100-565] - FFPE section of human ovarian carcinoma. Affinity purified rabbit anti-FUS used at a dilution of 1:1,000 (1 ug/mL). Detection: DAB
Immunohistochemistry-Paraffin: FUS Antibody [NB100-565] - FFPE section of mouse renal cells carcinoma. Affinity purified rabbit anti-FUS used at a dilution of 1:1,000 (1 ug/mL). Detection: DA
Western Blot: FUS Antibody [NB100-565] - Whole cell lysate (50 ug) from HepG2, HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Affinity purified rabbit anti-FUS antibody used for WB at 0.04 ug/mL. Detection: chemiluminescence with an exposure time of 10 seconds.
(4 Reviews)
(24 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, KO, WB
LC3A Antibody -
BSA Free
NB100-2331
Western Blot: LC3A Antibody - BSA Free [NB100-2331] - LC3A Antibody [NB100-2331] - Autophagy mobilized during differentiation of immortalized human mesenchymal stem cells (ihMSCs). Cultured ihMSCs were stimulated to undergo osteogenesis to form osteoblasts for 30 days at 21% and 4% oxygen. LC3I and LC3II levels were assessed via immunoblot every 10 days and normalized to beta-actin to derive average LC3II band densities. Image collected and cropped by CiteAb from the following publication (http://stemcellres.com/content/5/6/140), licensed under a CC-BY licence.
Western Blot: LC3A Antibody - BSA Free [NB100-2331] - LC3A Antibody [NB100-2331] - This LC3A antibody Image shows Analysis in human cell lysates.
Immunocytochemistry/Immunofluorescence: LC3A Antibody - BSA Free [NB100-2331] - LC3A Antibody [NB100-2331] - This LC3A antibody Image shows an analysis in HeLa cells using anti-LC3 antibody (red). Nuclei were counterstained with DAPI (blue).
(22 Reviews)
(279 Publications)
Species: Am, Ca, Fi, Hu, Mu, Pl, Rt, Ze
Applications: ChIP, ELISA, Flow, IB, ICC/IF, IHC-WhMt, IHC, IHC-Fr, IHC-P, IP, Simple Western, SB, WB
Ubiquilin 1
Antibody
NB120-3341
Ubiquilin 1 Antibody
Species: Hu, Mu, ChHa, Ha
Applications: WB

Publications for Ubiquilin 1 Antibody (NB120-3341) (0)

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Review for Ubiquilin 1 Antibody (NB120-3341) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB120-3341:
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Western Blot Ubiquilin 1 NB120-3341
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reviewed by:
Verified Customer
 WB Mouse 09/29/2010
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Summary

ApplicationWestern Blot
Sample Testedmouse cortical lysate, Sample Amount: 20ug
SpeciesMouse
Lotkk138754

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Western Blot Ubiquilin 1 NB120-3341
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 Verified Customer
09/29/2010
Application: WB
Species: Mouse
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Bioinformatics

Gene Symbol UBQLN1
Entrez