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Ubiquilin 1 Antibody

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Western Blot: Ubiquilin 1 Antibody [NB120-3341] - Analysis of ubiquilin on CHO cell extract.
Western Blot: Ubiquilin 1 Antibody [NB120-3341] - Analysis of ubiquilin on CHO cell extract.

Product Details

Summary
Reactivity Hu, Mu, ChHa, HaSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

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Ubiquilin 1 Antibody Summary

Immunogen
Synthetic Peptide: A(2) E S A E S G G P P G A Q D S A A(18)
Specificity
Ubiquilin 1
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
UBQLN1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
Application Notes
WB: Detects an approx. 63 kDa protein representing Ubiquilin from CHO cell extract.
Control
Ubiquilin 1 Knockout HeLa Cell Lysate
Reviewed Applications
Read 1 Review rated 4
using
NB120-3341 in the following application:

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 16945923).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 1 mg/ml BSA
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Ubiquilin 1 Antibody

  • DA41
  • DA41FLJ90054
  • DSK2
  • hPLIC-1
  • PLIC1
  • PLIC-1
  • PLIC-1UBQN
  • Protein linking IAP with cytoskeleton 1
  • Ubiquilin 1
  • ubiquilin-1
  • UBQLN1
  • UBQN
  • XDRP1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: beta Amyloid Antibody (MOAB-2) [NBP2-13075] - Immunofluorescent detection of beta Amyloid with MOAB-2 in the subiculim of 1-, 2- and 4- month old 5xFAD mice. Scale bar 20 um (from PMID: 22423893).
Western Blot: beta Amyloid Antibody (MOAB-2) [NBP2-13075] - Analysis of beta Amyloid (MOAB-2) antibody in (1) 100 pmole beta Amyloid 42, (2) 5xFAD mouse brain homogenate Repetition 1 and (3) 5xFAD mouse brain homogenate Repetition 2.
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Western Blot: Presenilin-1 Antibody (APS 18) [NB100-74510] - Analysis of THP-1 cell lysate.
Immunocytochemistry/Immunofluorescence: Presenilin-1 Antibody (APS 18) [NB100-74510] - Analysis of Presenilin 1 using Presenilin 1 Monoclonal antibody (APS 18) shows staining in A2058 melanoma cells. Presenilin 1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Presenilin 1 at a dilution of 1:20-1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
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Western Blot: S5a/Angiocidin Antibody [NBP2-37888] - Analysis in control (vector only transfected HEK293T lysate) and PSMD4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
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Applications: WB, ICC/IF, IHC, IHC-P
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Western Blot: Ubiquilin 2 Antibody (5F5) [H00029978-M03] - Generation of transgenic mice that overexpress human ubiquilin-1. Immunoblots of equal amounts of total brain lysates from 12 month-old WT mouse, 12 month-old Ubqln-1 48 transgenic mouse, 12 month-old Ubqln-1 62 transgenic mouse and end stage 15 week-old R6/2 transgenic mouse. The top panel was probed with a monoclonal anti-ubiquilin antibody (Invitrogen antibody clone 3D5E2) and the lower panel with a different monoclonal anti-Ubiquilin 2 antibody (Novus antibody clone 5F5). Note two immunoreactive ubiquilin bands are seen at 70 kDa and at 90 kDa, which we presume is a modified form of ubiquilin. Both blots were also probed for actin to ensure equal loading. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0087513) licensed under a CC-BY licence.
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Immunocytochemistry/Immunofluorescence: Ubiquilin 2 Antibody (5F5) [H00029978-M03] - Analysis of monoclonal antibody to UBQLN2 on A-431 cell. Antibody concentration 10 ug/ml
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Immunocytochemistry/Immunofluorescence: Presenilin-2 Antibody (APS 21) [NB100-74512] - Presenilin- 2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Presenilin 2 at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
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Flow Cytometry: Rad23 Antibody (3E3F4) [NBP2-52549] - Analysis of HeLa cells using RAD23A mouse mAb (green) and negative control (red).
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Flow Cytometry: Proteasome 19S 10B Antibody [NB100-1483] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Immunohistochemistry-Paraffin: DDI1 Antibody [NBP1-31417] - Paraffin-embedded OVCA, using antibody at 1:100 dilution.
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Immunohistochemistry-Paraffin: Utrophin Antibody [NBP1-84074] - Staining of human prostate shows moderate to strong membranous positivity in glandular cells.
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TFF1/pS2 Antibody
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(1 Review)
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DAN/NBL1 Antibody [Unconjugated]
(1 Publication)
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Applications: WB, Block
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(3 Reviews)
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Western Blot: LC3A Antibody [NB100-2331] - High autophagosome concentration is consumed during early immortalized human mesenchymal stem cell differentiation. Immortalized human mesenchymal stem cells were differentiated under osteogenic conditions (see Materials and methods) and assayed for changes in LC3I and LC3II during a 72-hour window. Cells were differentiated under standard conditions (top) or with addition of 5 uM rapamycin (middle) or 5 nM bafilomycin (bottom) for the first 3 hours of differentiation to modulate autophagy. Immunoblots were performed for LC3 at the indicated time points to assess autophagosome degradation via relative changes in LC3II (lower band; 17 kDa). Studies were repeated three times with similar trends seen consistently. Image collected and cropped by CiteAb from the following publication (http://stemcellres.com/content/5/6/140), licensed under a CC-BY licence.
Immunocytochemistry/Immunofluorescence: LC3A Antibody [NB100-2331] - Analysis in PFA fixed NIH/3T3 cells using anti-LC3A antibody. Image from verified customer review.
Immunohistochemistry: LC3A Antibody [NB100-2331] - Staining in mouse meniscus and cartilage. Image from verified customer review.
(20 Reviews)
(244 Publications)
Species: Hu, Mu, Rt, Ca, Fi, Pl, Ze
Applications: WB, Simple Western, ELISA, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, SB, IHC-WhMt
Ubiquilin 1
Antibody
NB120-3341
Ubiquilin 1 Antibody
Species: Hu, Mu, ChHa, Ha
Applications: WB

Publications for Ubiquilin 1 Antibody (NB120-3341) (0)

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Review for Ubiquilin 1 Antibody (NB120-3341) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB120-3341:
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Western Blot Ubiquilin 1 NB120-3341
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reviewed by:
Stephen Smith
WB Mouse 09/29/2010
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Summary

ApplicationWestern Blot
Sample Testedmouse cortical lysate, Sample Amount: 20ug
SpeciesMouse
Lotkk138754
CommentsGives a nice 75kDa band on western blot. 20ug mouse cortical lysate. Diluted 1:1000. Incubated overnight in 4% milk.

Blocking

Blocking DetailsBlocking Buffer: 5% milk, Blocking Time: 1 hour, Blocking Temp: room temperature

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Incubation Dilution Buffer: 5% milk in PBS/T, Incubation Time: overnight, Incubation Temp: 4C

Secondary Antibody

Secondary DescriptionSecondary Ab: biot-anti-rabbit, Secondary Ab Dilution Ratio: 1:10K

Details

Detection NotesDetection Method: Chemiluminescence, Specific Bands: 75 kDa

Comments

CommentsGives a nice 75kDa band on western blot. 20ug mouse cortical lysate. Diluted 1:1000. Incubated overnight in 4% milk.

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FAQs for Ubiquilin 1 Antibody (NB120-3341) (0)

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Control Lysate(s)

Ubiquilin 1 Knockout HeLa Cell Lysate

Secondary Antibodies

 

Isotype Controls

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Western Blot Ubiquilin 1 NB120-3341
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 Stephen Smith
09/29/2010
Application: WB
Species: Mouse
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Bioinformatics

Gene Symbol UBQLN1
Entrez