Ubiquilin 1 Antibody

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Western Blot: Ubiquilin 1 Antibody [NB120-3341] - Analysis of ubiquilin on CHO cell extract.

Product Details

Summary
Reactivity Hu, Mu, ChHa, HaSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Ubiquilin 1 Antibody Summary

Immunogen
Synthetic Peptide: A(2) E S A E S G G P P G A Q D S A A(18)
Specificity
Ubiquilin 1
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
UBQLN1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
Application Notes
WB: Detects an approx. 63 kDa protein representing Ubiquilin from CHO cell extract.
Control
Ubiquilin 1 Knockout HeLa Cell Lysate
Reviewed Applications
Read 1 Review rated 4
using
NB120-3341 in the following application:

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 16945923).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 1 mg/ml BSA
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Ubiquilin 1 Antibody

  • DA41
  • DA41FLJ90054
  • DSK2
  • hPLIC-1
  • PLIC1
  • PLIC-1
  • PLIC-1UBQN
  • Protein linking IAP with cytoskeleton 1
  • Ubiquilin 1
  • ubiquilin-1
  • UBQLN1
  • UBQN
  • XDRP1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB120-3341
Species: Hu, Mu, ChHa, Ha
Applications: WB

Publications for Ubiquilin 1 Antibody (NB120-3341) (0)

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Review for Ubiquilin 1 Antibody (NB120-3341) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB120-3341:
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WB
(1)
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Images Ratings Applications Species Date Details
Western Blot Ubiquilin 1 NB120-3341
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reviewed by:
Stephen Smith
WB Mouse 09/29/2010
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Summary

ApplicationWestern Blot
Sample Testedmouse cortical lysate, Sample Amount: 20ug
SpeciesMouse
Lotkk138754
CommentsGives a nice 75kDa band on western blot. 20ug mouse cortical lysate. Diluted 1:1000. Incubated overnight in 4% milk.

Blocking

Blocking DetailsBlocking Buffer: 5% milk, Blocking Time: 1 hour, Blocking Temp: room temperature

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Incubation Dilution Buffer: 5% milk in PBS/T, Incubation Time: overnight, Incubation Temp: 4C

Secondary Antibody

Secondary DescriptionSecondary Ab: biot-anti-rabbit, Secondary Ab Dilution Ratio: 1:10K

Details

Detection NotesDetection Method: Chemiluminescence, Specific Bands: 75 kDa

Comments

CommentsGives a nice 75kDa band on western blot. 20ug mouse cortical lysate. Diluted 1:1000. Incubated overnight in 4% milk.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Ubiquilin 1 Antibody (NB120-3341) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Recent Reviews

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5
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4
1
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1
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Stephen Smith
09/29/2010
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol UBQLN1
Entrez