UBE2S Antibody

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Western Blot: UBE2S Antibody [NBP2-20788] - Sample (30 ug of whole cell lysate) A: HCT116 12% SDS PAGE gel, diluted at 1:1000.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ca, RM, XpSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

UBE2S Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human UBE2S. The exact sequence is proprietary.
Predicted Species
Mouse (99%), Rat (99%), Canine (100%), Xenopus (99%), Rhesus Macaque (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
UBE2S
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
Theoretical MW
24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NBP2-20788 in the following application:

Reactivity Notes

Xenopus laevis (99%), Zebrafish (81%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for UBE2S Antibody

  • E2-24K
  • E2-EPF
  • E2-EPF5
  • E2-EPF52
  • E2-EPFEC 6.3.2.19
  • E2EPFEPF5
  • EPF5
  • UBE2S
  • Ubiquitin carrier protein S
  • ubiquitin-conjugating enzyme E2 S
  • ubiquitin-conjugating enzyme E2-24 kD
  • Ubiquitin-conjugating enzyme E2-24 kDa
  • Ubiquitin-conjugating enzyme E2-EPF5
  • ubiquitin-conjugating enzyme E2S
  • Ubiquitin-protein ligase S

Background

This gene encodes a member of the ubiquitin-conjugating enzyme family. The encoded protein is able to form a thiol ester linkage with ubiquitin in a ubiquitin activating enzyme-dependent manner, a characteristic property of ubiquitin carrier proteins. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for UBE2S Antibody (NBP2-20788) (0)

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Review for UBE2S Antibody (NBP2-20788) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP2-20788:
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WB
(1)
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Images Ratings Applications Species Date Details
Western Blot UBE2S NBP2-20788
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reviewed by:
Anonymous
WB Human 02/17/2016
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Summary

ApplicationWestern Blot
Sample TestedHela whole cell lysate
SpeciesHuman
Lot40296

Blocking

Blocking Details5% milk, 1 hour, room temperature

Primary Anitbody

Dilution Ratio1:800 in 5% BSA, incubate at 4 degree overnight

Secondary Antibody

Secondary DescriptionAnti-rabbit IgG (H+L) Dylight 800
Secondary Manufacturer Cat#cell signaling #5151
Secondary Concentration1:10000 dilution

Details

Detection NotesLI-COR Odyssey Infared Imaging system

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for UBE2S Antibody (NBP2-20788). (Showing 1 - 1 of 1 FAQ).

  1. I am interested in your Ube2s antibody, NBP2-20788, and would like to clarify what the immunogen is. You specify that the immuogen is a Recombinant fragment corresponding to a region within amino acids 1 and 185 of UBE2S. Does this mean this whole fragment comprising amino acids 1-185 was used as an immunogen or are you referring to a smaller region within residues 1-185? If the latter is true, what exactly is the region within 1-185 that was used as immunogen?
    • The immunogen given as 1-185 is just the amino acid range for the actual immunogen. The exact sequence is proprietary. If you would like us to help you align a specific sequence, we would be happy to do that for you.

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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5
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4
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1
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Anonymous
02/17/2016
Application: WB
Species: Human

Bioinformatics

Gene Symbol UBE2S
Entrez
Uniprot