UBE2S Antibody - BSA Free

Western Blot: UBE2S Antibody [NBP2-20788] - Wild-type (WT) and UBE2S knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with UBE2S antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: UBE2S Antibody [NBP2-20788] - Sample (30 ug of whole cell lysate) A: HCT116 12% SDS PAGE gel, diluted at 1:1000.

• Reactivity: Hu, Mu, Rt, Bv, Ca, RM, Xp
• Applications: WB, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

UBE2S Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human UBE2S. The exact sequence is proprietary.
• Predicted Species: Mouse (99%), Rat (99%), Canine (100%), Xenopus (99%), Rhesus Macaque (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: UBE2S
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Knockout Validated
  • Western Blot 1:500-1:3000
• Theoretical MW: 24 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (99%), Zebrafish (81%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for UBE2S Antibody - BSA Free

• E2-24K
• E2-EPF
• E2-EPF5
• E2-EPF52
• E2-EPFEC 6.3.2.19
• E2EPFEPF5
• EPF5
• UBE2S
• Ubiquitin carrier protein S
• ubiquitin-conjugating enzyme E2 S
• ubiquitin-conjugating enzyme E2-24 kD
• Ubiquitin-conjugating enzyme E2-24 kDa
• Ubiquitin-conjugating enzyme E2-EPF5
• ubiquitin-conjugating enzyme E2S
• Ubiquitin-protein ligase S

Background

This gene encodes a member of the ubiquitin-conjugating enzyme family. The encoded protein is able to form a thiol ester linkage with ubiquitin in a ubiquitin activating enzyme-dependent manner, a characteristic property of ubiquitin carrier proteins. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Review for UBE2S Antibody (NBP2-20788) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 02/17/2016

Summary

• Application: Western Blot
• Sample Tested: Hela whole cell lysate
• Species: Human
• Lot: 40296

FAQs for UBE2S Antibody (NBP2-20788). (Showing 1 - 1 of 1 FAQ).

1. I am interested in your Ube2s antibody, NBP2-20788, and would like to clarify what the immunogen is. You specify that the immuogen is a Recombinant fragment corresponding to a region within amino acids 1 and 185 of UBE2S. Does this mean this whole fragment comprising amino acids 1-185 was used as an immunogen or are you referring to a smaller region within residues 1-185? If the latter is true, what exactly is the region within 1-185 that was used as immunogen?
   • The immunogen given as 1-185 is just the amino acid range for the actual immunogen. The exact sequence is proprietary. If you would like us to help you align a specific sequence, we would be happy to do that for you.

Bioinformatics

• Gene Symbol: UBE2S
• Uniprot:
  • Q16763(Human)