UBE2M/Ubc12 Antibody (3C4) - Azide and BSA Free

Western Blot: UBE2M/Ubc12 Antibody (3C4) [H00009040-M01] - UBE2M monoclonal antibody (M01), clone 3C4 Analysis of UBE2M expression in Jurkat.

Immunocytochemistry/Immunofluorescence: UBE2M/Ubc12 Antibody (3C4) [H00009040-M01] - Analysis of monoclonal antibody to UBE2M on HeLa cell. Antibody concentration 10 ug/ml.

Sandwich ELISA: UBE2M/Ubc12 Antibody (3C4) [H00009040-M01] - Detection limit for recombinant GST tagged UBE2M is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 3C4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

UBE2M/Ubc12 Antibody (3C4) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: UBE2M (AAH58924, 1 a.a. ~ 183 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MIKLFSLKQQKKEEESAGGTKGSSKKASAAQLRIQKDINELNLPKTCDISFSDPDDLLNFKLVICPDEGFYKSGKFVFSFKVGQGYPHDPPKVKCETMVYHPNIDLEGNVCLNILREDWKPVLTINSIIYGLQYLFLEPNPEDPLNKEAAEVLQNNRRLFEQNVQRSMRGGYIGSTYFERCLK
• Specificity: UBE2M - ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: UBE2M
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for UBE2M/Ubc12 Antibody (3C4) - Azide and BSA Free

• EC 6.3.2
• EC 6.3.2.-
• hUbc12
• NEDD8 carrier protein
• NEDD8 protein ligase
• UBC12
• UBC12NEDD8-conjugating enzyme Ubc12
• UbcH12
• UBC-RS2
• UBE2M
• ubiquitin carrier protein M
• Ubiquitin-conjugating enzyme E2 M
• ubiquitin-conjugating enzyme E2M (homologous to yeast UBC12)
• ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast)
• ubiquitin-protein ligase M
• yeast UBC12 homolog

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for UBE2M/Ubc12 Antibody (H00009040-M01)(1)

Publication using H00009040-M01

• Miller BW, Lau G, Grouios C et al. Application of an integrated physical and functional screening approach to identify inhibitors of the Wnt pathway. Molecular Systems Biology. 2009-01-01 [PMID: 19888210]

Bioinformatics

• Gene Symbol: UBE2M
• Entrez:
  • 9040(Human)
• OMIM:
  • 603173(Human)
• Uniprot:
  • AAH58924(Human)