UBE2F/NCE2 Antibody [Alexa Fluor® 405]

• Reactivity: Hu
• Applications: ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Alexa Fluor 405

UBE2F/NCE2 Antibody [Alexa Fluor® 405] Summary

• Immunogen: Produced in rabbits immunized with purified, recombinant Human UBE2F/NCE2 (Uniprot#: Q969M7-1; Met1-Arg185)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: UBE2F
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Antigen and protein A Affinity-purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for UBE2F/NCE2 Antibody [Alexa Fluor® 405]

• EC 6.3.2
• EC 6.3.2.-
• MGC18120
• NCE2
• NCE2Ubiquitin-conjugating enzyme E2 F
• NEDD8 carrier protein UBE2F
• NEDD8 conjugating enzyme
• NEDD8 protein ligase UBE2F
• NEDD8-conjugating enzyme 2
• NEDD8-conjugating enzyme UBE2F
• UBE2F
• ubiquitin-conjugating enzyme E2F (putative)

Background

NCE2 is a ubiquitin conjugating enzyme. Ubiquitin is a 76 amino acid highly conserved eukaryotic polypeptide that selectively marks cellular proteins for proteolytic degradation by the 26S proteasome. The process of target selection, covalent attachment and shuttle to the 26S proteasome is a vital means of regulating the concentrations of key regulatory proteins in the cell by limiting their lifespans. Polyubiquitination is a common feature of this modification. Serial steps for modification include the activation of ubiquitin, an ATP dependent formation of a thioester bond between ubiquitin and the enzyme E1, transfer by transacylation of ubiquitin from E1 to the ubiquitin conjugating enzyme E2, and covalent linkage to the target protein directly by E2 or via E3 ligase enzyme. Deubiquitination enzymes also exist to reverse the marking of protein substrates. Posttranslational tagging by Ub is involved in a multitude of cellular processes, including the cell cycle, cell growth and differentiation, embryogenesis, apoptosis, signal transduction, DNA repair, regulation of transcription and DNA replication, transmembrane transport, stress responses, the immune response, and nervous system functions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: UBE2F