UBE2F/NCE2 Antibody

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Western Blot: UBE2F/NCE2 Antibody [NBP2-19485] - Sample (30 ug of whole cell lysate) A: NT2D1 12% SDS PAGE gel, diluted at 1:1000.

Product Details

Summary
Reactivity Hu, Mu, Bv, Ch, PmSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

UBE2F/NCE2 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human UBE2F/NCE2. The exact sequence is proprietary.
Predicted Species
Mouse (96%), Bovine (97%), Chicken (92%), Monkey (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
UBE2F
Purity
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rhesus Monkey (100%), Rat (83%), Zebrafish (81%), Xenopus laevis (88%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for UBE2F/NCE2 Antibody

  • EC 6.3.2
  • EC 6.3.2.-
  • MGC18120
  • NCE2
  • NCE2Ubiquitin-conjugating enzyme E2 F
  • NEDD8 carrier protein UBE2F
  • NEDD8 conjugating enzyme
  • NEDD8 protein ligase UBE2F
  • NEDD8-conjugating enzyme 2
  • NEDD8-conjugating enzyme UBE2F
  • UBE2F
  • ubiquitin-conjugating enzyme E2F (putative)

Background

Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Bioinformatics

Gene Symbol UBE2F
Uniprot