UbcH7/UBE2L3 Antibody - BSA Free

Images

 
Western Blot: UbcH7/UBE2L3 Antibody [NB100-2265] - Detection of Human and Mouse UBCH7 one HeLa whole cell lysate using NB100-2265.
Immunohistochemistry-Paraffin: UbcH7/UBE2L3 Antibody [NB100-2265] - FFPE section of human prostate carcinoma. Affinity purified rabbit anti- UBCH7 antibody used at a dilution of 1:1000 (1 ug/mL). Detection: DAB.
Immunohistochemistry-Paraffin: UbcH7/UBE2L3 Antibody [NB100-2265] - Human breast tumors stained with UbcH7 antibody. IHC-P image submitted by a verified customer review.

Product Details

Summary
Reactivity Hu, Mu, Bv, Pm-OrSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

UbcH7/UBE2L3 Antibody - BSA Free Summary

Description
Novus Biologicals Rabbit UbcH7/UBE2L3 Antibody - BSA Free (NB100-2265) is a polyclonal antibody validated for use in IHC, WB and IP. Anti-UbcH7/UBE2L3 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
Immunogen
The immunogen recognized by this antibody maps to a region between residue 100 and the C-terminus (residue 154) of human Ubiquitin-Conjugating Enzyme H7 using the numbering given in entry NP_003338.1 (GeneID 7332).
Predicted Species
Bovine (100%), Orangutan (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
UBE2L3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:1000 - 1:5000
  • Immunohistochemistry-Paraffin 1:1000 - 1:5000
  • Immunoprecipitation 2 - 5 ug / mg lysate
  • Western Blot 1:2000 - 1:10000
Application Notes
This UbcH7/UBE2L3 antibody is validated for IHC-P from a verified customer review. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. Immunoprecipitation: efficiency in IP is low.
Control
UbcH7/UBE2L3 Overexpression Lysate
Reviewed Applications
Read 1 Review rated 5
using
NB100-2265 in the following applications:

Publications
Read Publications using
NB100-2265 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for UbcH7/UBE2L3 Antibody - BSA Free

  • E2-F1
  • EC 6.3.2.19
  • L-UBC
  • UBCE7
  • UbcH7
  • UBCH7UbcM4
  • UbcM4
  • UBE2L3
  • Ubiquitin carrier protein L3
  • ubiquitin-conjugating enzyme E2 L3
  • Ubiquitin-conjugating enzyme E2-F1
  • ubiquitin-conjugating enzyme E2L 3
  • ubiquitin-conjugating enzyme UBCH7
  • Ubiquitin-protein ligase L3

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for UbcH7/UBE2L3 Antibody (NB100-2265)(3)

Review for UbcH7/UBE2L3 Antibody (NB100-2265) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-2265:
Filter by Applications
ICC
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry UbcH7/UBE2L3 NB100-2265
Enlarge
5
reviewed by:
Verified Customer
ICC Human 01/11/2016
View

Summary

ApplicationImmunocytochemistry
SpeciesHuman
Lotvarious

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Verified Customer
01/11/2016
Application: ICC
Species: Human

Bioinformatics

Gene Symbol UBE2L3
Entrez
Uniprot