UBE2G2 Antibody (OTI1B10)

Western Blot: UBE2G2 Antibody (OTI1B10) [NBP2-46278] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2G2.

Immunohistochemistry: UBE2G2 Antibody (OTI1B10) [NBP2-46278] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: UBE2G2 Antibody (OTI1B10) [NBP2-46278] - Analysis of Adenocarcinoma of Human colon tissue (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: UBE2G2 Antibody (OTI1B10) [NBP2-46278] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: UBE2G2 Antibody (OTI1B10) [NBP2-46278] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: UBE2G2 Antibody (OTI1B10) [NBP2-46278] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: UBE2G2 Antibody (OTI1B10) [NBP2-46278] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: UBE2G2 Antibody (OTI1B10) [NBP2-46278] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: UBE2G2 Antibody (OTI1B10) [NBP2-46278] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: UBE2G2 Antibody (OTI1B10) [NBP2-46278] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunofluorescence: UBE2G2 Antibody (OTI1B10) [NBP2-46278] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY UBE2G2.

• Reactivity: Hu, Mu, Rt, Ca, Pm
• Applications: WB, ICC/IF, IHC
• Clone: OTI1B10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

UBE2G2 Antibody (OTI1B10) Summary

• Immunogen: Full length human recombinant protein of human UBE2G2(NP_003334) produced in HEK293T cell.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: UBE2G2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:200-2000
• Theoretical MW: 18.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for UBE2G2 Antibody (OTI1B10)

• EC 6.3.2.19
• UBC7
• UBC7human ubiquitin conjugating enzyme G210ubiquitin-conjugating enzyme E2G 2 (homologous to yeast UBC7)
• UBE2G2
• Ubiquitin carrier protein G2
• ubiquitin conjugating enzyme 7
• ubiquitin conjugating enzyme G2
• ubiquitin-conjugating enzyme E2 G2
• ubiquitin-conjugating enzyme E2G 2 (UBC7 homolog, yeast)
• Ubiquitin-protein ligase G2

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein shares 100% sequence identity with the mouse counterpart. This gene is ubiquitously expressed, with high expression seen in adult muscle. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: UBE2G2