U2AF35 Antibody (1E9) - BSA Free

Immunohistochemistry: U2AF35 Antibody (1E9) [NBP3-43059] - Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using (U2AF35 Antibody) at dilution of 1:50

Immunoprecipitation: U2AF35 Antibody (1E9) [NBP3-43059] - IP Result of anti-U2AF35 (IP:FNab09138, 3ug; Detection:FNab09138 1:500) with HeLa cells lysate 3000ug.

Western Blot: U2AF35 Antibody (1E9) [NBP3-43059] - HeLa cells were subjected to SDS PAGE followed by western blot with (U2AF35 Antibody) at dilution of 1:1000

• Reactivity: Hu
• Applications: WB, ELISA, IHC, IP
• Clone: 1E9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

U2AF35 Antibody (1E9) - BSA Free Summary

• Description: Novus Biologicals Mouse U2AF35 Antibody (1E9) - BSA Free (NBP3-43059) is a monoclonal antibody validated for use in IHC, WB, ELISA and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: U2 small nuclear RNA auxiliary factor 1 - (Uniprot# Q01081)
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: U2AF1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 1:20 - 1:200
  • Immunoprecipitation 1:200 - 1:2000
  • Western Blot 1:500 - 1:5000
• Theoretical MW: 38 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for U2AF35 Antibody (1E9) - BSA Free

• FP793
• human U2 snRNP auxiliary factor small subunit10U2AF35DKFZp313J1712
• RN
• RNU2AF1,35-KD subunit
• U2 auxiliary factor 35 kDa subunit
• U2 small nuclear RNA auxiliary factor 1
• U2 small nuclear RNA auxillary factor 1
• U2 snRNP auxiliary factor small subunit

Background

U2AF35 belongs to the splicing factor SR family of genes. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: U2AF1