u-Plasminogen Activator/Urokinase Antibody (U-6) - BSA Free

Product Discontinued

u-Plasminogen Activator/Urokinase Antibody (U-6) - BSA Free Summary

• Immunogen: Native human uPA
• Epitope: Clone U-6 binds to the A-chain of uPA
• Specificity: Specific for human uPA. No reaction with human tissue plasminogen activator (tPA) is seen when tested by ELISA, immunoblotting and enzyme inhibition. No reaction with any other human plasma proteins is seen when tested by immunoblotting. Bbinds single and two-chain uPA, uPA/PAI-1 complex and uPA/uPAR complex. A reaction is seen with the amino terminal fragment (ATF) but not with LMW uPA.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PLAU
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen
  • Western Blot 1:100-1:2000
• Application Notes: ELISA: NBP2-23504 can be used in ELISA. (1, 2, 3) WB: NBP2-23504 reacts with reduced and non-reduced uPA in Western blotting. It inhibits the enzymatic activity of uPA in the fibrin plate assay. IHC: NBP2-23504 can be used on frozen sections in immunohistochemistry applications. (4)

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 10mM Phosphate (pH 7.4) and 0.14M NaCl
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for u-Plasminogen Activator/Urokinase Antibody (U-6) - BSA Free

• ATF
• EC 3.4.21
• EC 3.4.21.73
• plasminogen activator, urokinase
• PLAU
• uPA
• u-PA
• uPlasminogen Activator
• u-Plasminogen Activator
• urinary
• Urokinase
• urokinase-type plasminogen activator

Background

Urokinase plasminogen activator (uPA) is a serine protease. It converts the abundant proenzyme plasminogen to active plasmin and plays a key role in cancer invasion and a variety of tissue remodelling processes such as wound healing, mammary gland involution and placental development (1-3). Elevated levels of uPA are associated with poor prognosis in many types of cancer (2-5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PLAU