Recombinant Human TWEAK/TNFSF12 Protein

Product Discontinued

Recombinant Human TWEAK/TNFSF12 Protein Summary

• Description: Recombinant bioactive protein for Human TWEAK/TNFSF12
  
  

  Amino Acid Sequence: (O43508)

• Preparation Method: Determined to be >98% pure by SDS-PAGE
• Details of Functionality: The ED50 as determined by the dose-dependent stimulation of IL-8 production by human PBMC is less than 10 ng/ml.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: TNFSF12
• Purity: >98%, by SDS-PAGE
• Endotoxin Note: <0.1 ng/ug

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 17 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: Sterile filtered and lyophilized from 10 mM Sodium Phosphate (pH 7.5), 0.1 M L-Arg.
• Concentration: Lyoph
• Purity: >98%, by SDS-PAGE
• Reconstitution Instructions: Reconstitute in 10 mM Sodium Phosphate (pH 7.5) to a concentration of 1 mg/ml. This solution can then be diluted into other aqueous buffers.

Alternate Names for Recombinant Human TWEAK/TNFSF12 Protein

• APO3 ligand
• APO3LMGC20669
• DR3LGTWEAKAPO3/DR3 ligand
• MGC129581
• TNF-related weak inducer of apoptosis
• TNFSF12
• tumor necrosis factor (ligand) superfamily, member 12
• tumor necrosis factor ligand superfamily member 12
• TWEAK

Background

Human TWEAK is a new secreted ligand in the tumor necrosis factor family that weakly induces apoptosis. TWEAK, like many other members of the TNF family, is able to induce interleukin-8 synthesis in a number of cell lines. The recombinant human TWEAK is a 17.0 kDa protein, comprising the TNF-like extraCellular domain of TWEAK

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: TNFSF12