Mouse TWEAK/TNFSF12 ELISA Kit (Colorimetric) Summary
| Description |
Quality control test: Standard Curve. Tnfsf12 (Mouse) ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Tnfsf12. |
| Sensitivity |
0.3 pg/mL |
| Assay Type |
Sandwich ELISA |
| Inter-Assay |
CV% < 12% |
| Intra-Assay |
CV% < 10% |
| Sample Volume |
100 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
TNFSF12 |
Applications/Dilutions
| Dilutions |
|
| Application Notes |
Useful for quantification. |
Packaging, Storage & Formulations
| Storage |
Store at -20 to -80C. Avoid freeze-thaw cycles. |
Kit Components
|
Components
|
- Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For detection antibody and HRP-Streptavidin diluent.
- Assay Diluent C (Item L): 30 ml of diluent buffer. For Standard/ Sample (serum/plasma samples/cell culture medium) diluent.
- Detection Antibody Tweak (Item F): 2 vial of biotinylated anti-mouse Tweak (each vial is enough to assay half microplate).
- HRP-Streptavidin Concentrate (Item G): 8 µl 10,000x concentrated HRP-conjugated streptavidin.
- Standards (Item C): 2 vials of recombinant mouse Tweak.
- Stop Solution (Item I): 8 ml of 2 M sulfuric acid.
- TMB One-Step Substrate Reagent (Item H): 12 ml of 3,3’,5,5’-tetramethylbenzidine (TMB) in buffered solution.
- Tweak Microplate (Item A): 96 wells (12 strips x 8 wells) coated with anti-mouse Tweak.
- Wash Buffer Concentrate (20x) (Item B): 25 ml of 20x concentrated solution.
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Mouse TWEAK/TNFSF12 ELISA Kit (Colorimetric)
Background
CD266 is a 14 kD transmembrane protein known as TWEAK receptor (TWEAK-R) or fibroblast growth factor inducible 14 (Fn14). It is member 12A of the tumor necrosis factor receptor superfamily (TNFRSF12A) expressed at low levels in a variety of tissues, such as heart, placenta, lung, muscle, and pancreas. TWEAK-R binds TWEAK (CD255) and interacts with the cytoplasmic proteins TRAF1, 2, and 3. The engagement of Fn14 can induce cell death and may play a role in inflammation, hepatocyte growth, and liver neoplasia. ITEM-4 antibody reacts with both mouse and human Fn14. It is useful for flow cytometric analysis.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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FAQs for TWEAK/TNFSF12 ELISA Kit (KA1818). (Showing 1 - 1 of 1 FAQ).
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wondering what the difference is between your quantikine and duo set elisas?
- Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.
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