Mouse TWEAK/TNFSF12 ELISA Kit (Colorimetric)

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Summary
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    • Catalog Number
      KA1818
    • Availability
      Product Discontinued

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Mouse TWEAK/TNFSF12 ELISA Kit (Colorimetric) Summary

Description
Quality control test: Standard Curve. Tnfsf12 (Mouse) ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Tnfsf12.
Sensitivity
0.3 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 12%
Intra-Assay
CV% < 10%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
TNFSF12

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
Useful for quantification.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.

Kit Components

Components
  1. Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For detection antibody and HRP-Streptavidin diluent.
  2. Assay Diluent C (Item L): 30 ml of diluent buffer. For Standard/ Sample (serum/plasma samples/cell culture medium) diluent.
  3. Detection Antibody Tweak (Item F): 2 vial of biotinylated anti-mouse Tweak (each vial is enough to assay half microplate).
  4. HRP-Streptavidin Concentrate (Item G): 8 µl 10,000x concentrated HRP-conjugated streptavidin.
  5. Standards (Item C): 2 vials of recombinant mouse Tweak.
  6. Stop Solution (Item I): 8 ml of 2 M sulfuric acid.
  7. TMB One-Step Substrate Reagent (Item H): 12 ml of 3,3’,5,5’-tetramethylbenzidine (TMB) in buffered solution.
  8. Tweak Microplate (Item A): 96 wells (12 strips x 8 wells) coated with anti-mouse Tweak.
  9. Wash Buffer Concentrate (20x) (Item B): 25 ml of 20x concentrated solution.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Mouse TWEAK/TNFSF12 ELISA Kit (Colorimetric)

  • APO3 ligand
  • APO3LMGC20669
  • DR3LGTWEAKAPO3/DR3 ligand
  • MGC129581
  • TNF-related weak inducer of apoptosis
  • TNFSF12
  • tumor necrosis factor (ligand) superfamily, member 12
  • tumor necrosis factor ligand superfamily member 12
  • TWEAK

Background

CD266 is a 14 kD transmembrane protein known as TWEAK receptor (TWEAK-R) or fibroblast growth factor inducible 14 (Fn14). It is member 12A of the tumor necrosis factor receptor superfamily (TNFRSF12A) expressed at low levels in a variety of tissues, such as heart, placenta, lung, muscle, and pancreas. TWEAK-R binds TWEAK (CD255) and interacts with the cytoplasmic proteins TRAF1, 2, and 3. The engagement of Fn14 can induce cell death and may play a role in inflammation, hepatocyte growth, and liver neoplasia. ITEM-4 antibody reacts with both mouse and human Fn14. It is useful for flow cytometric analysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for TWEAK/TNFSF12 ELISA Kit (KA1818). (Showing 1 - 1 of 1 FAQ).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

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Bioinformatics

Gene Symbol TNFSF12