TWEAK/TNFSF12 Antibody

Recombinant Mouse TWEAK/TNFSF12 (Catalog # 1237-TW) inhibits proliferation in the the HT‑29 human colon adenocarcinoma cell line in a dose-dependent manner (orange line). Activity elicited by Recombinant Mouse TWEAK/TNFSF12 (2 µg/mL) is neutralized (green line) by increasing concen­trations of Goat Anti-Mouse TWEAK/TNFSF12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1237). The ND50 is typically 4-16 µg/mL.

• Reactivity: Mu
• Applications: WB, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

TWEAK/TNFSF12 Antibody Summary

• Immunogen: E. coli-derived recombinant mouse TWEAK/TNFSF12
  Arg105-His249
  Accession # O54907
• Specificity: Detects TWEAK/TNFSF12 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross‑reactivity with recombinant human TWEAK is observed and less than 1% cross-reactivity with recombinant mouse (rm) BAFF, rmFas Ligand, rmOX40 Ligand, rmTNF-alpha , and rmTRAIL is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: Tnfsf12
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 4-16 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TWEAK/TNFSF12 Antibody

• APO3 ligand
• APO3LMGC20669
• DR3LGTWEAKAPO3/DR3 ligand
• MGC129581
• TNF-related weak inducer of apoptosis
• TNFSF12
• tumor necrosis factor (ligand) superfamily, member 12
• tumor necrosis factor ligand superfamily member 12
• TWEAK

Background

TNF-related weak inducer of apoptosis (TWEAK) is a type II transmembrane protein belonging to the TNF superfamily and has been designated TNFSF12. Mouse TWEAK is a 249 amino acid (aa) protein with an N-terminal 21 aa cytoplasmic domain, a 21 aa transmembrane region and a 204 aa C-terminal extracellular domain (1). The primary structures of the extracellular domains of human and mouse TWEAK are 88% identical. A soluble form of TWEAK is generated from the membrane-associated molecules by proteolytic cleavage suggesting that TWEAK may have long-range effects. TWEAK is expressed widely in many tissues and cells (1). Although TWEAK has been proposed as a ligand that signals through the death domain receptor 3 (DR3) (2), a TNF receptor superfamily member currently designated TNFRSF25, subsequent studies did not demonstrate binding of TWEAK to cell lines that express DR3 (3). In cells that lack DR3, TWEAK has been shown to bind TWEAK receptor (TWEAK R), a novel TNF receptor superfamily member designated TNFRSF12A (4‑7). TWEAK R, also known as fibroblast growth factor-inducible 14 (Fn14), is a growth factor-inducible immediate-early response gene that is expressed in fibroblasts, hepatocellular carcinomas and endothelial cells. TWEAK-TWEAK R interaction has been shown to promote NF-kappa B activation and mediate multiple cell death pathways. On endothelial cells, TWEAK R plays a role in endothelial cell growth and migration. This effect of TWEAK is not due to upregulation of VEGF (8).

1. Chicheportiche, Y. et al. (1997) J. Biol Chem. 272:32401.
2. Marsters, S. et al. (1998) Current Biol. 8:525.
3. Kaptein, A. et al. (2000) FEBS Letters, 485:135.
4. Schneider, P. et al. (1999) Eur. J. Immunol. 29:1785.
5. Nakayama, M. et al. (2002) J. Immunol. 168:734.
6. Feng, S.L. et al. (2000) Am. J. Pathol. 156:1253.
7. Wiley, S.R. et al. (2001) Immunity 15:837.
8. Lynch, C. et al. (1998) J. Biol. Chem. 274:8455.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TWEAK/TNFSF12 Antibody (AF1237)(2)

Publications using AF1237

• Lucas Cheadle, Samuel A. Rivera, Jasper S. Phelps, Katelin A. Ennis, Beth Stevens, Linda C. Burkly et al. Sensory Experience Engages Microglia to Shape Neural Connectivity through a Non-Phagocytic Mechanism Neuron 2020-11-01 [PMID: 32931754]
• Lucas Cheadle, Samuel A. Rivera, Jasper S. Phelps, Katelin A. Ennis, Beth Stevens, Linda C. Burkly et al. Sensory Experience Engages Microglia to Shape Neural Connectivity through a Non-Phagocytic Mechanism Neuron 2020-11-01 [PMID: 32931754] (Immunohistochemistry, Mouse)

Bioinformatics

• Gene Symbol: Tnfsf12
• Uniprot:
  • O54907()