TWA1 Antibody

Images

 
Biological Strategies: Western Blot: TWA1 Antibody [NBP1-32596] - ARMC8 HEK293 CRISPR knockout cells were analyzed by Western blot with antibodies to CTLH complex members, as indicated. Vinculin was used as a ...read more
Immunocytochemistry/ Immunofluorescence: TWA1 Antibody [NBP1-32596] - Paraformaldehyde-fixed HeLa, using antibody at 1:1000 dilution.
Immunohistochemistry-Paraffin: TWA1 Antibody [NBP1-32596] - Paraffin-embedded Cal27 xenograft, using antibody at 1:500 dilution.
Western Blot: TWA1 Antibody [NBP1-32596] - (30 ug whole cell lysate) A: 293T 12% SDS PAGE; antibody diluted at 1:1000
Genetic Strategies: Knockout Validated: TWA1 Antibody [NBP1-32596] - TWA1 HEK293 CRISPR knockout cells were analyzed by Western blot with antibodies to CTLH complex members, as indicated. Vinculin was used as a ...read more

Product Details

Summary
Reactivity Hu, Rt, Mu, Bv, Ch, RM, XpSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

TWA1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human C20orf11. The exact sequence is proprietary.
Localization
Nucleus
Predicted Species
Mouse (99%), Rhesus Macaque (100%), Bovine (97%), Xenopus (96%), Chicken (97%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GID8
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Knockout Validated
  • Western Blot 1:500-1:3000
Application Notes
IHC-Fr (Frozen sections) Assay dependent
Theoretical MW
27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP1-32596 in the following applications:

  • WB
    2 publications

Reactivity Notes

Xenopus laevis (96%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for TWA1 Antibody

  • bA305P22.1
  • C20orf11
  • chromosome 20 open reading frame 11
  • FLJ20602
  • GID complex subunit 8 homolog (S. cerevisiae)
  • Twa1
  • TWA1protein C20orf11
  • two hybrid associated protein 1
  • Two hybrid-associated protein 1 with RanBPM

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, KO
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for TWA1 Antibody (NBP1-32596)(3)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GID8