Tubulin Antibody (B3)

Product Discontinued

Tubulin Antibody (B3) Summary

• Immunogen: Purified sea urchin (Lytechinus pictus) sperm axonemal proteins.
• Epitope: The epitope recognized by the antibody is localized in the C-terminal region of alpha and beta tubulins (the glutamylated motif at amino acid 445-457 of alpha tubulin).
• Localization: Centrosomal.
• Marker: Microtubule Marker
• Specificity: Tubulin - Polyglutamylated (B3)
• Isotype: IgM
• Clonality: Monoclonal
• Host: Mouse
• Gene: TUBA1A
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1-2 ug/ml
• Application Notes: ICC: Can be used with 4% paraformaldehyde-1% Triton X-100 and methanol-acetone.WB: The concentration is recommended using a cytosolic fraction of rat brain extract. Detects a band of approximately 50 kDa, possibly a doublet of alpha and beta tubulins.

Reactivity Notes

Cross-reacts with Human, Mouse, Rat, Cow, Chicken, Macaque Monkey, Rabbit and Sea Urchin. Not yet tested in other species. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS (pH 7.4) and 1.0% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 2.1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Tubulin Antibody (B3)

• Alpha-tubulin 3
• B-ALPHA-1
• FLJ25113
• hum-a-tub2
• LIS3
• TUBA3hum-a-tub1
• tubulin alpha-1A chain
• Tubulin alpha-3 chain
• Tubulin B-alpha-1
• tubulin, alpha 1a
• tubulin, alpha 3
• tubulin, alpha, brain-specific

Background

Tubulins comprise an important family of globular proteins, which commonly form microtubules for cytoskeltal structure. The most common Tubulin isoforms are alpha, beta and gamma tubulin. Alpha Tubulin is a fairly ubiquitous microtubule component, whereas beta Tubulin is specific to neuronal microtubules. Gamma Tubulin is primarily found in centrosomes and spindle pole bodies, where it is critical for proper nucleation and polar orientation of microtubules. The final tubulin family members, delta and episilon Tubulin, are less well studied but thought to be associated with mitotic spindle formation. Tubulin antibodies are very useful as controls for protein standarization and in both cytoskeletan and cell cycle studies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Tubulin Antibody (NB120-11324). (Showing 1 - 1 of 1 FAQ).

1. I am interested in buying a Tubulin antibody to use in Western blot as a loading control. We would like to use it on rat heart samples. Can you make a recommendation?
   • I would recommend the Tubulin Antibody (YL1/2) catalog # NB600-506. This antibody works great as a loading control in Western blot and has been validated for use with rat samples.

Bioinformatics

• Gene Symbol: TUBA1A
• Entrez:
  • 7846(Human)
  • 22142(Mouse)
  • 64158(Rat)