Tubulin alpha-1B Antibody

Immunohistochemistry-Paraffin: Tubulin alpha-1B Antibody [NBP2-20759] - Immunohistochemical analysis of paraffin-embedded U251 xenograft, using antibody at 1:500 dilution.

Western Blot: Tubulin alpha-1B Antibody [NBP2-20759] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Tubulin alpha-1B antibody (NBP2-20759) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: Tubulin alpha-1B Antibody [NBP2-20759] - Various whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Tubulin alpha-1B antibody (NBP2-20759) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry: Tubulin alpha-1B Antibody [NBP2-20759] - Tubulin alpha-1B antibody detects Tubulin alpha-1B protein on zebrafish by whole mount immunohistochemical analysis. Sample: 2 days-post-fertilization zebrafish embryo. Tubulin alpha-1B antibody (NBP2-20759) dilution: 1:200.

Immunocytochemistry/ Immunofluorescence: Tubulin alpha-1B Antibody [NBP2-20759] - Tubulin alpha-1B antibody detects Tubulin alpha-1B protein at cytoskeleton by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Tubulin alpha-1B protein stained by Tubulin alpha-1B antibody (NBP2-20759) diluted at 1:1000.Blue: Hoechst 33342 staining.Scale bar = 10 um.

Immunohistochemistry: Tubulin alpha-1B Antibody [NBP2-20759] - Immunohistochemical analysis (whole mount) of zebrafish embryo, using Tubulin alpha-1B antibody (NBP2-20759) at 1:200 dilution.

Western Blot: Tubulin alpha-1B Antibody [NBP2-20759] - Various tissue extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Tubulin alpha-1B antibody (NBP2-20759) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry: Tubulin alpha-1B Antibody [NBP2-20759] - Tubulin alpha-1B antibody detects Tubulin alpha-1B protein on zebrafish by whole mount immunohistochemical analysis. Sample: Paraformaldehyde-fixed 2 day-post-fertilization zebrafish embryo.Tubulin alpha-1B antibody (NBP2-20759) dilution: 1:200.

alpha Tubulin antibody detects alpha Tubulin protein at axons on whole-mount zebrafish embryos by immunohistochemical analysis. Sample: Paraformaldehyde-fixed zebrafish embryos. alpha Tubulin antibody (NBP2-20759) dilution: 1:200.

alpha Tubulin antibody detects alpha Tubulin protein at cytoskeleton by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: alpha Tubulin stained by alpha Tubulin antibody (NBP2-20759) diluted at 1:500.Blue: Fluoroshield with DAPI .

• Reactivity: Hu, Mu, Rt, Ze, Po, Bv, Pm, Pm, Rb, Xp, Ze
• Applications: WB, ICC/IF, IHC, WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Tubulin alpha-1B Antibody Summary

• Description: Novus Biologicals Rabbit Tubulin alpha-1B Antibody (NBP2-20759) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Tubulin alpha-1B. The exact sequence is proprietary.
• Marker: Microtubule Marker
• Predicted Species: Porcine (100%), Bovine (100%), Rabbit (100%), Zebrafish (96%), Primate (100%), Monkey (100%), Xenopus (95%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TUBA1B
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoblotting
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:20000
• Application Notes: Immunoblotting Assay-dependent dilution
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rhesus Monkey (100%), Chimpanzee (100%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Tubulin alpha-1B Antibody

• alpha tubulin
• Alpha-tubulin ubiquitous
• K-ALPHA-1
• tubulin alpha-1B chain
• Tubulin alpha-ubiquitous chain
• Tubulin K-alpha-1
• tubulin, alpha 1b
• tubulin, alpha, ubiquitous

Background

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TUBA1B