TTK Antibody

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Western Blot: TTK Antibody [NB100-462] - Detection of Human and Mouse TTK by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, and mouse TCMK-1 cells. Antibodies: Affinity purified rabbit ...read more
Western Blot: TTK Antibody [NB100-462] - Detection of human TTK in HeLa cells treated with an siRNA for vimentin (control) or for TTK (siTTK), using NB 100-462.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
   

Biological Strategies

 

Genetic Strategies

   

Order Details

TTK Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 700 and 750 of human TTK using the numbering given Swiss-Prot in entry P33981 (GeneID 7272).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TTK
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000 - 1:5000
  • Immunoprecipitation 1 - 4 ug/mg lysate
Application Notes
NB 100-462 may be used for Western Blot and immunoprecipitation. Suggested working dilutions: * Western Blot - 1:2,500-1:25,000 (ECL) Immunocytochemistry - Not Determined Immunoprecipitation - 1-4 ug/mg lysate *The investigator should determine the optimal working dilution for a specific application.
Positive Control
TTK Lysate (NBL1-17415)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TTK Antibody

  • CT96
  • dual specificity protein kinase TTK
  • EC 2.7.12.1
  • ESK
  • FLJ38280
  • monopolar spindle 1-like 1
  • Mps1
  • MPS1L1
  • MPS1L1PYTcancer/testis antigen 96
  • phosphotyrosine picked threonine kinase
  • Phosphotyrosine picked threonine-protein kinase
  • PYT
  • TTK protein kinase
  • TTK

Background

This 95 kDa protein phosphorylates other proteins on serine, threonine, and tyrosine. It has been thought to be associated with cell proliferation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TTK
Entrez
Uniprot