TSPAN7/TM4SF2 Antibody (482618) [Alexa Fluor® 647]

HEK293 human embryonic kidney cell line transfected with either (A) human TSPAN7 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human TSPAN7 Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB9179R). Quadrant markers were set based on control antibody staining (Catalog # IC003R). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 482618
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 647

TSPAN7/TM4SF2 Antibody (482618) [Alexa Fluor® 647] Summary

• Immunogen: HEK293 human embryonic kidney cell line transfected with human TSPAN7
  Accession # P41732
• Specificity: Stains human TSPAN7 transfectants but not irrelevant transfectants in flow cytometry.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 5 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TSPAN7/TM4SF2 Antibody (482618) [Alexa Fluor® 647]

• A15
• A15CCG-B7
• CCG-B7
• CD231 antigen
• CD231
• Cell surface glycoprotein A15
• DXS1692E
• Membrane component chromosome X surface marker 1
• mental retardation, X-linked 58
• MRX58
• MXS1
• MXS1T-cell acute lymphoblastic leukemia associated antigen 1
• TALLA-1
• T-cell acute lymphoblastic leukemia-associated antigen 1
• tetraspanin 7
• tetraspanin-7
• TM4SF2
• TM4SF2b
• TM4SF2tetraspanin protein
• transmembrane 4 superfamily 2b
• Transmembrane 4 superfamily member 2X chromosome, surface marker 1
• transmembrane protein A15
• TSPAN7
• tspan-7

Background

Tetraspanin-7 (aka TSPAN7,  TALLA-1, TM4SF2 and CD231) is a 249 aminoacids (aa) multi-pass membrane protein.  Tetraspanin proteins regulate morphology, signaling and trafficking processes by interaction and association with proteins in tetraspanin enriched microdomains (TEMs). It has been proposed that tetraspanins interact with integrins to affect cell migration, probably by modulation or compartmentalization of integrin signaling. TSPAN7 has been found to be upregulated in myeloma development and described as a prognostic marker of renal cell carcinoma. Mutations in TSPAN7 are also implicated in some forms of X-linked intellectual disability.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TSPAN7/TM4SF2 Antibody (FAB9179R)(1)

Publication using FAB9179R

• L Man, N Lustgarten, E Kallinos, Z Redhead-La, S Liu, B Schattman, D Redmond, K Hancock, N Zaninovic, G Schattman, Z Rosenwaks, D James Comparison of Human Antral Follicles of Xenograft versus Ovarian Origin Reveals Disparate Molecular Signatures Cell Rep, 2020-08-11;32(6):108027. 2020-08-11 [PMID: 32783948] (Flow Cytometry, ICC, IHC, Human)

Bioinformatics

• Uniprot:
  • P41732(Human)