Tryptophanyl tRNA synthetase Antibody (3A12) - Azide and BSA Free

Western Blot: Tryptophanyl tRNA synthetase Antibody (3A12) [H00007453-M02] - WARS monoclonal antibody (M02), clone 3A12 Analysis of WARS expression in HeLa.

Immunocytochemistry/Immunofluorescence: Tryptophanyl tRNA synthetase Antibody (3A12) [H00007453-M02] - Analysis of monoclonal antibody to WARS on HeLa cell. Antibody concentration 10 ug/ml.

Western Blot: Tryptophanyl tRNA synthetase Antibody (3A12) [H00007453-M02] - Analysis of WARS expression in transfected 293T cell line by WARS monoclonal antibody (M02), clone 3A12.Lane 1: WARS transfected lysate(53.2 KDa).Lane 2: Non-transfected lysate.

Immunoprecipitation: Tryptophanyl tRNA synthetase Antibody (3A12) [H00007453-M02] - Analysis of WARS transfected lysate using anti-WARS monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with WARS MaxPab rabbit polyclonal antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IP
• Clone: 3A12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Tryptophanyl tRNA synthetase Antibody (3A12) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: WARS (NP_004175, 50 a.a. ~ 149 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. YKAAAGEDYKADCPPGNPAPTSNHGPDATEAEEDFVDPWTVQTSSAKGIDYDKLIVRFGSSKIDKELINRIERATGQRPHHFLRRGIFFSHRDMNQVLDA
• Specificity: WARS (3A12)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: WARS1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
  • Western Blot
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Tryptophanyl tRNA synthetase Antibody (3A12) - Azide and BSA Free

• EC 6.1.1
• EC 6.1.1.2
• GAMMA-2
• hWRS
• IFI53
• IFP53trpRS
• Interferon-induced protein 53
• TrpRS
• tryptophan tRNA ligase 1, cytoplasmic
• Tryptophan--tRNA ligase
• tryptophanyl-tRNA synthetase
• tryptophanyl-tRNA synthetase, cytoplasmic
• WRS

Background

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of tryptophanyl-tRNA synthetase exist, a cytoplasmic form, named WARS, and a mitochondrial form, named WARS2. Tryptophanyl-tRNA synthetase (WARS) catalyzes the aminoacylation of tRNA(trp) with tryptophan and is induced by interferon. Tryptophanyl-tRNA synthetase belongs to the class I tRNA synthetase family. Four transcript variants encoding two different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: WARS1
• Entrez:
  • 7453(Human)
• OMIM:
  • 191050(Human)
• Uniprot:
  • NP_004175(Human)