Tryptophan hydroxylase 2 Antibody

Immunocytochemistry/Immunofluorescence: Tryptophan hydroxylase 2 Antibody [NB100-2447] - Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Immunohistochemistry-Paraffin: Tryptophan hydroxylase 2 Antibody [NB100-2447] - Staining of paraffin embedded Human Brain Stem. Antibody at 2 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Flow Cytometry: Tryptophan hydroxylase 2 Antibody [NB100-2447] - Flow cytometric analysis of paraformaldehyde fixed U251 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Immunocytochemistry/Immunofluorescence: Tryptophan hydroxylase 2 Antibody [NB100-2447] - Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

• Reactivity: Hu, Po, Bv, Ca
• Applications: ELISA, Flow, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Tryptophan hydroxylase 2 Antibody Summary

• Description: Novus Biologicals Goat Tryptophan hydroxylase 2 Antibody (NB100-2447) is a polyclonal antibody validated for use in IHC, ELISA, Flow and ICC/IF. Anti-Tryptophan hydroxylase 2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-NKPNSGKNDDKGNK corresponding to internal region according to NP_775489.2.
• Predicted Species: Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TPH2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/mL
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Immunohistochemistry 2 - 4 ug/mL
  • Immunohistochemistry-Paraffin 2 - 4 ug/mL
  • Peptide ELISA Detection limit 1:32000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Tryptophan hydroxylase 2 Antibody

• ADHD7
• EC 1.14.16
• EC 1.14.16.4
• FLJ37295
• MGC138872
• Neuronal tryptophan hydroxylase
• NTPH
• NTPHMGC138871
• TPH2
• tryptophan 5-hydroxylase 2
• Tryptophan 5-monooxygenase 2
• Tryptophan Hydroxylase 2

Background

Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II) (Jiang et al., 2000; Johansen et al., 1996). CaM K II phosphorylates Ser260 which lies within the regulatory domain of TPH (Jiang et al., 2000).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Tryptophan hydroxylase 2 Antibody (NB100-2447)(2)

Publications using NB100-2447

• Zhou Z, Roy A, Lipsky R et al. Haplotype-based linkage of tryptophan hydroxylase 2 to suicide attempt, major depression, and cerebrospinal fluid 5-hydroxyindoleacetic acid in 4 populations. Arch Gen Psychiatry 2005-10-01 [PMID: 16203956]
• Jansch C Effects of SLC2A3 copy number variants on neurodevelopment and glucose metabolism in ADHD patient-specific neurons Thesis 2021-01-01 (ICC/IF, Human)

Bioinformatics

• Gene Symbol: TPH2
• Entrez:
  • 121278(Human)
• Uniprot:
  • NP_775489.2(Human)