Tryptophan Hydroxylase 1/TPH-1 Overexpression Lysate (Native)

Images

 
Western Blot: Tryptophan Hydroxylase 1 Overexpression Lysate (Adult Normal) [NBL1-17216] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Tryptophan Hydroxylase 1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Tryptophan Hydroxylase 1/TPH-1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC221254 and based on accession number NM_004179. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens tryptophan hydroxylase 1 (TPH1), mRNA.
Gene
TPH1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
50.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Tryptophan Hydroxylase 1/TPH-1

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Tryptophan Hydroxylase 1/TPH-1 Overexpression Lysate (Native)

  • EC 1.14.16
  • EC 1.14.16.4
  • indoleacetic acid-5-hydroxylase
  • L-tryptophan hydroxylase
  • MGC119994
  • TPH
  • TPH-1
  • TPRH
  • TRPH
  • tryptophan 5-hydroxylase 1
  • Tryptophan 5-monooxygenase 1
  • tryptophan hydroxylase (tryptophan 5-monooxygenase)
  • Tryptophan Hydroxylase 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-74555
Species: Hu, Mu, Rt, Pm, Rb
Applications: WB, ICC/IF, IHC, IHC-P, IP, IHC-FrFl
H00006532-M06
Species: Hu
Applications: ELISA, IP
NBP2-45995
Species: Hu
Applications: WB, IHC
NB300-109
Species: Hu, Mu, Rt, Dr, I, Ma
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P, IHC-FrFl
NBP2-01752
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-52084
Species: Hu, Mu, Rt
Applications: WB, IHC-P, PEP-ELISA
NBP1-85818
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP2-26091
Species: Hu
Applications: WB, IHC, IHC-P, PEP-ELISA
NBP2-21590
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NB100-135
Species: Hu, Pm, Bv(-), Ch(-), Rt(-), Ze(-)
Applications: WB, ICC/IF
NBP1-81838
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NB300-174
Species: Hu, Mu, Rt, Bv
Applications: WB, Simple Western, IHC, IHC-P
H00001815-R02
Species: Hu
248-BD/CF
Species: Hu, Mu, Rt, Ca, Eq
MAB5764
Species: Hu
Applications: Flow, IHC, CyTOF-ready
NB100-56350
Species: Hu, Mu, Rt
Applications: WB
NBP2-43801
Species: Hu
Applications: WB
AF682
Species: Hu
Applications: WB, IHC, ELISA(Cap), ELISA(Det), Neut, ELISA(Sta)
NBL1-17216
Species: Hu
Applications: WB

Publications for Tryptophan Hydroxylase 1/TPH-1 Lysate (NBL1-17216) (0)

There are no publications for Tryptophan Hydroxylase 1/TPH-1 Lysate (NBL1-17216).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Tryptophan Hydroxylase 1/TPH-1 Lysate (NBL1-17216) (0)

There are no reviews for Tryptophan Hydroxylase 1/TPH-1 Lysate (NBL1-17216). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Tryptophan Hydroxylase 1/TPH-1 Lysate (NBL1-17216) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Tryptophan Hydroxylase 1/TPH-1 Products

Bioinformatics Tool for Tryptophan Hydroxylase 1/TPH-1 Lysate (NBL1-17216)

Discover related pathways, diseases and genes to Tryptophan Hydroxylase 1/TPH-1 Lysate (NBL1-17216). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Tryptophan Hydroxylase 1/TPH-1 Lysate (NBL1-17216)

Discover more about diseases related to Tryptophan Hydroxylase 1/TPH-1 Lysate (NBL1-17216).
 

Pathways for Tryptophan Hydroxylase 1/TPH-1 Lysate (NBL1-17216)

View related products by pathway.

PTMs for Tryptophan Hydroxylase 1/TPH-1 Lysate (NBL1-17216)

Learn more about PTMs related to Tryptophan Hydroxylase 1/TPH-1 Lysate (NBL1-17216).
 

Research Areas for Tryptophan Hydroxylase 1/TPH-1 Lysate (NBL1-17216)

Find related products by research area.

Blogs on Tryptophan Hydroxylase 1/TPH-1

There are no specific blogs for Tryptophan Hydroxylase 1/TPH-1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

BDNF [Unconjugated]
248-BD-005/CF

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Tryptophan Hydroxylase 1/TPH-1 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol TPH1
Entrez