Tryptophan Hydroxylase 1/TPH-1 Antibody - Azide Free

Western Blot: Tryptophan Hydroxylase 1/TPH-1 Antibody [NB300-176] - Western blot of human dorsal Raphe nucleus showing specifc immunolabeling of the ~55k tryptophan hydroxylase protein.

• Reactivity: Hu, Mu, Rt, Rb(-), Mu, Bv, Ca, Eq, Fe, Gt, Gp, Ha, Pm, Sh, Vo
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Format: Azide Free

Tryptophan Hydroxylase 1/TPH-1 Antibody - Azide Free Summary

• Description: Novus Biologicals Sheep Tryptophan Hydroxylase 1/TPH-1 Antibody - Azide Free (NB300-176) is a polyclonal antibody validated for use in IHC and WB. Anti-Tryptophan Hydroxylase 1/TPH-1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant rabbit tryptophan hydroxylase, isolated as inclusion bodies from E. coli and purified by preparative SDS-PAGE. Accession # P17290
• Specificity: Specific for endogenous levels of the ~55 kDa Tryptophan Hydroxylase 1/TPH-1 protein.
• Predicted Species: Mouse (100%), Feline (100%), Bovine (100%), Guinea Pig (100%), Primate (100%), Equine (100%), Vole (100%), Canine (100%), Goat (100%), Hamster (100%), Sheep (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: TPH1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:1000
  • Western Blot 1:1000
• Theoretical MW: 55 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Reactivity assumed based on sequence identity to a wide variety of mammalian species. Mouse reactivity reported in scientific literature (PMID: 28456683).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for Tryptophan Hydroxylase 1/TPH-1 Antibody - Azide Free

• EC 1.14.16
• EC 1.14.16.4
• indoleacetic acid-5-hydroxylase
• L-tryptophan hydroxylase
• MGC119994
• TPH
• TPH-1
• TPRH
• TRPH
• tryptophan 5-hydroxylase 1
• Tryptophan 5-monooxygenase 1
• tryptophan hydroxylase (tryptophan 5-monooxygenase)
• Tryptophan Hydroxylase 1

Background

Tryptophan hydroxylase (TPH) is one of a small family of monooxygenases that use tetrahydropterine as a cofactor during the catabolism of aromatic L-amino acids. It contains catalytic and regulatory domains, as well as a short tetramerization domain. TPH is the first catalytic step in the biosynthesis of serotonin in the CNS and melatonin in the pineal gland. TPH plays an important role in the pathology of several neuropsychiatric disorders, including depression, aggression, and schizophrenia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Tryptophan Hydroxylase 1/TPH-1 Antibody (NB300-176)(2)

Publications using NB300-176

• Qu C, Yuan ZW, Yu XT et al. Patchouli alcohol ameliorates dextran sodium sulfate-induced experimental colitis and suppresses tryptophan catabolism. Pharmacol. Res. 2017-04-26 [PMID: 28456683] (WB, Mouse)
• Haycock JW, Kumer SC, Lewis DA, Vrana KE, Stockmeier CA. A monoclonal antibody to tryptophan hydroxylase: applications and identification of the epitope. J. Neurosci. Methods. 114(2):205-12. 2002-01-01 [PMID: 11856572]

Bioinformatics

• Gene Symbol: TPH1
• Entrez:
  • 7166(Human)
  • 24848(Rat)
• Uniprot:
  • P17752()