Trypsin 3/PRSS3 Antibody (420012) [Alexa Fluor® 647]

• Reactivity: Hu
• Applications: WB
• Clone: 420012
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 647

Trypsin 3/PRSS3 Antibody (420012) [Alexa Fluor® 647] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Trypsin 3/PRSS3
  Val16-Ser247
  Accession # NP_002762
• Specificity: Detects human Trypsin 3/PRSS3 in direct ELISAs and Western blots. Detects both pro and mature forms of Trypsin 3 in Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Trypsin 1, 2, recombinant mouse Trypsin
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Trypsin 3/PRSS3 Antibody (420012) [Alexa Fluor® 647]

• Brain trypsinogen
• EC 3.4.21
• EC 3.4.21.4
• Mesotrypsin
• mesotrypsinogen
• MTG
• pancreatic trypsinogen III
• protease, serine, 3 (mesotrypsin)
• protease, serine, 3
• protease, serine, 4 (trypsin 4, brain)
• PRSS3
• PRSS4
• Serine protease 3
• Serine protease 4
• T9
• TRY3MTG
• TRY4mesotrypsin
• Trypsin 3
• Trypsin 4
• Trypsin III
• Trypsin IV
• trypsin-3
• Trypsinogen 15
• trypsinogen 4
• trypsinogen 5
• trypsinogen IV

Background

Human Trypsin 3, encoded by the PRSS3 gene, is also known as mesotrypsin (1). Constituting less than 10% of the total trypsin content in normal pancreatic juice, it is one of the three trypsin isoforms produced by the pancreas (2). Compared to Trypsin 1 and 2, one intriguing feature of Trypsin 3 is its resistance to polypeptide trypsin inhibitors, such as the Kunitz-type soybean trypsin inhibitor or the Kazal-type pancreatic secretory trypsin inhibitor. As revealed by the crystal structure, this resistance is likely due to the presence of an arginine residue in place of the highly conserved Gly198 (3). Trypsin 3 is synthesized in the pancreas and secreted into the duodenum lumen, where it is activated by enterokinase. One physiologic function of Trypsin 3 has been proposed to be degradation of trypsin inhibitors, which facilitates the digestion of those foods rich in these proteins (4).The amino acid sequence of human Trypsin 3 shares 76%, 74%, 72% and 67% identity with that of rat, mouse, dog and chicken, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.