Trypsin 3/PRSS3 Antibody (419911)

Trypsin 3/PRSS3 was detected in perfusion fixed frozen sections of mouse pancreas using 25 µg/mL Rat Anti-Mouse Trypsin 3/PRSS3 Monoclonal Antibody (Catalog # MAB3565) overnight at 4 °C. Tissue was stained with the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Trypsin 3/PRSS3 was detected in perfusion fixed frozen sections of mouse kidney using 25 µg/mL Rat Anti-Mouse Trypsin 3/ PRSS3 Monoclonal Antibody (Catalog # MAB3565) overnight at 4 °C. Tissue was stained with the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: IHC
• Clone: 419911
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Trypsin 3/PRSS3 Antibody (419911) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Trypsin 3/PRSS3
  Phe16-Asn246
  Accession # NP_035775
• Specificity: Detects mouse Trypsin 3/PRSS3 in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: PRSS3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Trypsin 3/PRSS3 Antibody (419911)

• Brain trypsinogen
• EC 3.4.21
• EC 3.4.21.4
• Mesotrypsin
• mesotrypsinogen
• MTG
• pancreatic trypsinogen III
• protease, serine, 3 (mesotrypsin)
• protease, serine, 3
• protease, serine, 4 (trypsin 4, brain)
• PRSS3
• PRSS4
• Serine protease 3
• Serine protease 4
• T9
• TRY3MTG
• TRY4mesotrypsin
• Trypsin 3
• Trypsin 4
• Trypsin III
• Trypsin IV
• trypsin-3
• Trypsinogen 15
• trypsinogen 4
• trypsinogen 5
• trypsinogen IV

Background

Mouse Trypsin 3, encoded by the PRSS3 gene, is also known as mesotrypsin. It consists of a signal peptide (residues 1 to 15), a pro region (residues 16 to 23), and a mature chain (residues 24 to 246) (1). The purified recombinant mouse Trypsin 3 corresponds to the pro form, which can be activated by enterokinase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRSS3
• Uniprot:
  • NP_035775()