Troponin T Type 2 (cardiac) Antibody (SC64-03)

Western Blot: Troponin T Type 2 (cardiac) Antibody (SC64-03) [NBP2-67594] - Analysis of Cardiac Troponin T Type 2 on rat heart tissue lysates with Rabbit anti-Cardiac Troponin T Type 2 antibody at 1/500 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 35 kDa Observed band size: 40/37 kDa Exposure time: 30 seconds; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry-Paraffin: Troponin T Type 2 (cardiac) Antibody (SC64-03) [NBP2-67594] - Analysis of paraffin-embedded rat heart tissue using anti-Cardiac Troponin T antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Western Blot: Troponin T Type 2 (cardiac) Antibody (SC64-03) [NBP2-67594] - Western blot analysis of Troponin T Type 2 (cardiac) on rat skeletal muscle tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room

Western Blot: Troponin T Type 2 (cardiac) Antibody (SC64-03) [NBP2-67594] - Analysis of Cardiac Troponin T Type 2 on mouse heart tissue lysates with Rabbit anti-Cardiac Troponin T Type 2 antibody at 1/500 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 35 kDa Observed band size: 40 kDa Exposure time: 1 minute; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry-Paraffin: Troponin T Type 2 (cardiac) Antibody (SC64-03) [NBP2-67594] - Analysis of mouse heart tissue using anti-Cardiac Troponin T antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Troponin T Type 2 (cardiac) Antibody (SC64-03) [NBP2-67594] - Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Troponin T Type 2 (cardiac) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-51, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: Troponin T Type 2 (cardiac) Antibody (SC64-03) [NBP2-67594] - Analysis of paraffin-embedded human striated muscle tissue using anti-Cardiac Troponin T Type 2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Product Discontinued

Troponin T Type 2 (cardiac) Antibody (SC64-03) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within human Troponin T Type 2 (cardiac) aa 251-298/298. (SwissProt: P45379 Human; SwissProt: P50752 Mouse; SwissProt: P50753 Rat)
• Localization: Cytoplasm, filament.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: TNNT2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Troponin T Type 2 (cardiac) Antibody (SC64-03)

• cardiac
• CMD1D
• CMPD2
• dilated 1D (autosomal dominant)
• hypertrophic 2
• MGC3889
• RCM3
• TnTc
• troponin T type 2 (cardiac)
• troponin T, cardiac muscle

Background

Does not cross-react with skeletal muscle troponin T, cTnI or TnC.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TNNT2