DMPK Antibody (713931)

Western blot shows lysates of human heart tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human DMPK Monoclonal Antibody (Catalog # MAB6967) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DMPK at approximately 74 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clone: 713931
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

DMPK Antibody (713931) Summary

• Immunogen: E. coli-derived recombinant human DMPK
  Glu393-Thr543
  Accession # Q09013
• Specificity: Detects human DMPK in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: DMPK
• Purity: Protein A or G purified from hybridoma culture supernatant
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A or G purified from hybridoma culture supernatant
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DMPK Antibody (713931)

• DM1
• DM1PK
• DMK
• DMKEC 2.7.11.1
• DM-kinase
• DMmyotonin protein kinase A
• DMPK
• dystrophia myotonica-protein kinase
• MDPK
• MDPKDM protein kinase
• MT-PK
• MT-PKmyotonic dystrophy associated protein kinase
• Myotonic dystrophy protein kinase
• myotonin-protein kinase
• thymopoietin homolog

Background

DMPK (dystrophia myotonica protein kinase), also called DMK, MDPK or MT-PK (myotonin protein kinase) is an 80 kDa cytoplasmic protein of the ser/thr kinase superfamily (EC 2.7.11.1). The 639 amino acid (aa) human DMPK includes a protein kinase domain (aa 81-349), an AGC kinase C-terminal domain (aa 350-425) and a coiled-coil negative regulatory domain that mediates dimerization (aa 467-546). Within the region used as an immunogen, human DMPK shares 72% and 73% aa sequence identity with mouse and rat DMPK, respectively. This region is included within 12 isoforms that range in size from 530-634 aa. The most prominent, muscle-specific isoform phosphorylates phospholamban and is critical for cardiac contractility.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for DMPK Antibody (MAB6967) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 01/09/2023

Summary

• Application: Western Blot
• Sample Tested: 293T whole cell lysate,Hela whole cell lysate
• Species: Human
• Lot: CFNG0221031

Bioinformatics

• Gene Symbol: DMPK
• Uniprot:
  • Q09013()