tropomyosin-4 Antibody (4E4-1D2) - Azide and BSA Free

Western Blot: tropomyosin-4 Antibody (4E4-1D2) [H00007171-M01] - TPM4 monoclonal antibody (M01), clone 4E4-1D2 Analysis of TPM4 expression in Hela.

Immunocytochemistry/Immunofluorescence: tropomyosin-4 Antibody (4E4-1D2) [H00007171-M01] - Analysis of monoclonal antibody to TPM4 on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: tropomyosin-4 Antibody (4E4-1D2) [H00007171-M01] - Analysis of monoclonal antibody to TPM4 on formalin-fixed paraffin-embedded human transitional cell carcinoma tissue. Antibody concentration 2 ug/ml.

Sandwich ELISA: tropomyosin-4 Antibody (4E4-1D2) [H00007171-M01] - Detection limit for recombinant GST tagged TPM4 is approximately 1ng/ml as a capture antibody.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 4E4-1D2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

tropomyosin-4 Antibody (4E4-1D2) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: TPM4 (AAH37576, 1 a.a. ~ 248 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAGLNSLEAVKRKIQALQQQADEAEDRAQGLQRELDGERERREKAEGDVAALNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIENRAMKDEEKMEIQEMQLKEAKHIAEEADRKYEEVARKLVILEGELERAEERAEVSELKCGDLEEELKNVTNNLKSLEAASEKYSEKEDKYEEEIKLLSDKLKEAETRAEFAERTVAKLEKTIDDLEEKLAQAKEENVGLHQTLDQTLNELNCI
• Specificity: TPM4 - tropomyosin 4
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TPM4
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for tropomyosin-4 Antibody (4E4-1D2) - Azide and BSA Free

• TM30p1
• tropomyosin 4
• tropomyosin alpha-4 chain
• tropomyosin-4

Background

The tropomyosin-4 gene encodes a member of the tropomyosin family of actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosins are dimers of coiled-coil proteins that polymerize end-

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for tropomyosin-4 Antibody (H00007171-M01)(2)

Publications using H00007171-M01

• Habig K, Gellhaar S, Heim B et al. LRRK2 guides the actin cytoskeleton at growth cones together with ARHGEF7 and Tropomyosin 4. Biochim Biophys Acta. 2013-09-24 [PMID: 24075941]
• Shankar J, Messenberg A, Chan J et al. Pseudopodial actin dynamics control epithelial-mesenchymal transition in metastatic cancer cells Cancer Res 2010-05-01 [PMID: 20388789] (WB, ICC/IF, Human)

Bioinformatics

• Gene Symbol: TPM4
• Entrez:
  • 7171(Human)
• OMIM:
  • 600317(Human)
• Uniprot:
  • AAH37576(Human)