TROP-2 Antibody (TACSTD2/2152)

Immunocytochemistry/Immunofluorescence: TROP-2 Antibody (TACSTD2/2152) [NBP2-75745] - Confocal Immunofluorescence of MCF-7 cells. TROP-2 Antibody (TACSTD2/2152). labeled with CF488 (Green); Red Dot is used to label the nuclei.

Immunohistochemistry-Paraffin: TROP-2 Antibody (TACSTD2/2152) [NBP2-75745] - Formalin-fixed, paraffin-embedded human Pancreatic Carcinoma stained with TACSTD2 / TROP2 Mouse Monoclonal Antibody (TACSTD2/2152).

Immunohistochemistry-Paraffin: TROP-2 Antibody (TACSTD2/2152) [NBP2-75745] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with TACSTD2 / TROP2 Mouse Monoclonal Antibody (TACSTD2/2152).

Immunohistochemistry-Paraffin: TROP-2 Antibody (TACSTD2/2152) [NBP2-75745] - Formalin-fixed, paraffin-embedded human Pancreatic Carcinoma stained with TACSTD2 / TROP2 Mouse Monoclonal Antibody (TACSTD2/2152).

Protein Array: TROP-2 Antibody (TACSTD2/2152) [NBP2-75745] - Analysis of Protein Array containing >19,000 full-length human proteins using TROP-2 Antibody (TACSTD2/2152) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, MA
• Clone: TACSTD2/2152
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

TROP-2 Antibody (TACSTD2/2152) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-75746)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
• Immunogen: Recombinant fragment of human TROP-2 protein (around aa 31-274) (exact sequence is proprietary) (Uniprot: P09758 )
• Localization: Cell Surface
• Marker: Epithelial Marker
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TACSTD2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Protein Array
  • Western Blot 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for TROP-2 Antibody (TACSTD2/2152)

• Cell surface glycoprotein Trop-2
• EGP1
• EGP-1
• epithelial glycoprotein-1
• GA733-1
• GA733-1GP50
• gastrointestinal tumor-associated antigen GA7331
• gp50
• M1S1cell surface glycoprotein TROP2
• Membrane component chromosome 1 surface marker 1
• membrane component, chromosome 1, surface marker 1
• Pancreatic carcinoma marker protein GA733-1
• pancreatic carcinoma marker protein GA7331,40kD glycoprotein, identified by monoclonal GA733
• T16
• TACSTD2
• TROP2
• TROP-2
• TROP2GA7331
• tumor-associated calcium signal transducer 2

Background

TROP2 encodes a carcinoma-associated antigen defined by the monoclonal antibody GA733. This antigen is a member of a family including at least two type I membrane proteins. It transduces an intracellular calcium signal and acts as a cell surface receptor. Mutations of this gene result in gelatinous drop-like corneal dystrophy, an autosomal recessive disorder characterized by severe corneal amyloidosis leading to blindness. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TACSTD2
• Uniprot:
  • P09758()
  • P09758()
  • P09758()