TrkC Overexpression Lysate

Product Discontinued

TrkC Overexpression Lysate Summary

• Immunogen: The lysate was created in HEK293T cells, using plasmid ID RC219113 and based on accession number NM_001012338. The protein contains a C-terminal DDK tag.
• Specificity: Homo sapiens neurotrophic tyrosine kinase, receptor, type 3 (NTRK3), transcript variant 1, mRNA.
• Gene: NTRK3

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This product is intended for use as a positive control in Western Blot.
  
  You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
• Theoretical MW: 90.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for TrkC Overexpression Lysate

• EC 2.7.10
• EC 2.7.10.1
• ETS related protein-neurotrophic receptor tyrosine kinase fusion protein
• ETV6-NTRK3 fusion
• gp145(trkC)
• GP145-TrkC
• Neurotrophic tyrosine kinase receptor type 3
• neurotrophic tyrosine kinase, receptor, type 3
• NT 3 receptor
• NT-3 growth factor receptor
• NTRK3
• TrkC tyrosine kinase
• TrkC
• trk-C
• TRKCneurotrophin 3 receptor
• tyrosine kinase receptor C

Background

TrkC (tyrosine kinase receptor C) is a receptor for neurotrophin-3. TrkC is a member of receptor tyrosine kinases and may have a role in proprioceptive neuron development. TrkC has been studied in relation to various diseases and disorders including leukemia, breast cancer, fibrosarcoma, Alzheimer's disease, Parkinson's disease and several others.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: NTRK3