Triosephosphate isomerase Antibody (2C3)

Western Blot: Triosephosphate isomerase Antibody (2C3) [H00007167-M02A] - Analysis of TPI1 expression in HepG2.

Product Discontinued

Triosephosphate isomerase Antibody (2C3) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: TPI1 (AAH07086.1, 1 a.a. ~ 249 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAPSRKFFVGGNWKMNGRKQSLGELIGTLNAAKVPADTEVVCAPPTAYIDFARQKLDPKIAVAAQNCYKVTNGAFTGEISPGMIKDCGATWVVLGHSERRHVFGESDELIGQKVAHALAEGLGVIACIGEKLDEREAGITEKVVFEQTKVIADNVKDWSKVVLAYEPVWAIGTGKTATPQQAQEVHEKLRGWLKSNVSDAVAQSTRIIYGGSVTGATCKELASQPDVDGFLVGGASLKPEFVDTINAKQ
• Specificity: TPI1 (2C3)
• Isotype: IgM Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TPI1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: No Preservative
• Purity: Unpurified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Triosephosphate isomerase Antibody (2C3)

• EC 5.3.1.1
• MGC88108
• TIM
• TPI
• triosephosphate isomerase 1
• triosephosphate isomerase
• Triose-phosphate isomerase

Background

Triosephosphate isomerase (TIM) catalyses the reversible interconversion of G3P and DHAP. Only G3P can be used in glycolysis, therefore TIM is essential for energy production, allowing two molecules of G3P to be produced for every glucose molecule, thereby doubling the energy yield. Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency) [MIM:190450]. TPI deficiency is an autosomal recessive disorder. It is the most severe clinical disorder of glycolysis. It is associated with neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Triosephosphate isomerase Antibody (H00007167-M02A)(1)

Publication using H00007167-M02A

• Smith L, Qutob O, Watson MB et al. Proteomic identification of putative biomarkers of radiotherapy resistance: a possible role for the 26S proteasome? Neoplasia. 2009-11-01 [PMID: 19881955]

Bioinformatics

• Gene Symbol: TPI1
• Entrez:
  • 7167(Human)
• OMIM:
  • 190450(Human)
• Uniprot:
  • AAH07086(Human)