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TRIM41 Antibody - BSA Free

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Immunohistochemistry-Paraffin: TRIM41 Antibody [NBP3-05293] - Analysis of a FFPE tissue section of human small intestine using 1:200 dilution of TRIM41 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.
Immunohistochemistry-Paraffin: TRIM41 Antibody [NBP3-05293] - Analysis of a FFPE tissue section of human small intestine using 1:200 dilution of TRIM41 antibody. The staining was developed using HRP labeled anti-rabbit ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP3-05293AF350
Alexa Fluor 405
NBP3-05293AF405
Alexa Fluor 488
NBP3-05293AF488
Alexa Fluor 532
NBP3-05293AF532
Alexa Fluor 594
NBP3-05293AF594
Alexa Fluor 647
NBP3-05293AF647
Alexa Fluor 700
NBP3-05293AF700
Alexa Fluor 750
NBP3-05293AF750
Biotin
NBP3-05293B
DyLight 350
NBP3-05293UV
DyLight 405
NBP3-05293V
DyLight 488
NBP3-05293G
DyLight 550
NBP3-05293R
DyLight 594
NBP3-05293DL594
DyLight 650
NBP3-05293C
DyLight 680
NBP3-05293FR
DyLight 755
NBP3-05293IR
FITC
NBP3-05293F
HRP
NBP3-05293H
Janelia Fluor 549
NBP3-05293JF549
Janelia Fluor 646
NBP3-05293JF646
View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP3-05293SS
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TRIM41 Antibody - BSA Free Summary

Immunogen
Partial synthetic peptide made to an internal portion of the human TRIM41 protein (between amino acids 400-470) [UniProt Q8WV44].
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TRIM41
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 5 - 10 ug/ml
  • Immunohistochemistry 1:100 - 1:300
  • Immunohistochemistry-Paraffin 1:100 - 1:300
  • Western Blot 1-2 ug/ml

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TRIM41 Antibody - BSA Free

  • tripartite motif containing 41

Background

TRIM41 belongs to a family of tripartite motif (TRIM) proteins defined as containing a RING finger, one or more B-boxdomains, and a coiled-coil region (Tanaka et al., 2005 (PubMed 16022281)). See TRIM45 (MIM 609318).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western blot shows lysates of human peripheral blood mononuclear cells and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TRIM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4708) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for TRIM at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Human CD3+ T cells from peripheral blood mononuclear cells were stained with Goat Anti-Human TRIM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4708, filled histo­gram) or isotype control antibody (Catalog #AB-108-C, open histo­gram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for TRIM at approximately 38 kDa (as indicated) using 20 µg/mL of Goat Anti-Human TRIM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4708) . This experiment was conducted under reducing conditions and using the 12-230kDa separation system.
Species: Hu
Applications: CyTOF-ready, ICFlow, Simple Western, WB
PRRT2 Antibody -
BSA Free
NBP2-82026
Western Blot: PRRT2 Antibody [NBP2-82026] - Analysis of PRRT2 in mouse brain tissue lysate with PRRT2 antibody at 1 ug/ml.
Immunohistochemistry: PRRT2 Antibody [NBP2-82026] - Immunohistochemistry of PRRT2 in rat brain tissue with PRRT2 antibody at 2.5 ug/ml.
Immunocytochemistry/Immunofluorescence: PRRT2 Antibody [NBP2-82026] - Immunofluorescence of PRRT2 in rat brain tissue with PRRT2 antibody at 20 ug/ml.
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
ZNF645 Antibody
NBP2-21037
Western Blot: ZNF645 Antibody [NBP2-21037] - Sample (30 ug of whole cell lysate) A: MCF-7 10% SDS PAGE gel, diluted at 1:500.
Immunohistochemistry-Paraffin: ZNF645 Antibody [NBP2-21037] - Immunohistochemical analysis of paraffin-embedded GASTRIC CA, using antibody at 1:500 dilution.
Species: Hu
Applications: IHC, IHC-P, WB
MUL1 Antibody
(5H6.2D5) - BSA Free
NBP2-31361
Western Blot: MUL1 Antibody (5H6.2D5) [NBP2-31361] - WB detection of partial recombinant protein MUL1 by using MUL1 antibody (clone 5H6.2D5) at a concentration of 0.01 ug/ml.
Immunohistochemistry-Paraffin: MUL1 Antibody (5H6.2D5) [NBP2-31361] - IHC-P analysis of MUL1 protein in a section of normal prostate from human using 5 ug/ml concentration of MUL1 antibody (clone 5H6.2D5). Strong cytoplasmic staining was observed in the epithelial columnar and basal cells of prostate glands.
Immunohistochemistry-Paraffin: MUL1 Antibody (5H6.2D5) [NBP2-31361] - IHC-P analysis of MUL1 protein in a section of normal cardiac stomach from human using 5 ug/ml concentration of MUL1 antibody (clone 5H6.2D5). The cardiac glands showed distinct cytoplasmic staining, wherein, the parietal cells as well as the chief cells were found positive for MUL1.
Species: Hu
Applications: IHC, IHC-P, WB
TRIM50 Antibody
NBP1-81988
Immunohistochemistry-Paraffin: TRIM50 Antibody [NBP1-81988] - Staining of human bone marrow shows strong cytoplasmic positivity in a subset of bone marrow poietic cells.
Species: Hu
Applications: IHC, IHC-P
JNK1 Antibody
(228601) [Unconjugate
MAB17761
Western blot shows lysates of CHP-100 human neuroblastoma cell line, C6 rat glioma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat JNK1 Monoclonal Antibody (Catalog # MAB17761) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for JNK1 was detected at approximately 46 kDa and 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
Western blot shows recombinant human JNK1, JNK2, and JNK3 (1 ng/lane). PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat JNK1 Monoclonal Antibody (Catalog # MAB17761) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for JNK1 was detected at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
JNK1 was detected in immersion fixed MCF‑7 human breast cancer cell line using Mouse Anti-Human/Mouse/Rat JNK1 Monoclonal Antibody (Catalog # MAB17761) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
(1 Review)
(3 Publications)
Species: Hu, Mu, Rt
Applications: ICC, WB
Sertad1 Antibody
NBP1-31052
Immunohistochemistry-Paraffin: Sertad1 Antibody [NBP1-31052] - Paraffin-embedded Adrenal gland tumor, using antibody at 1:100 dilution.
Western Blot: Sertad1 Antibody [NBP1-31052] - Sample (30 ug of whole cell lysate) A: Molt-4 12% SDS PAGE SERTAD1 antibody, antibody diluted at 1:1000.
Species: Hu
Applications: IHC, IHC-P, WB

beta-Glucuronidase/GUS
NBP1-87511
Immunocytochemistry/Immunofluorescence: beta-Glucuronidase/GUSB Antibody [NBP1-87511] - staining of human cell line U-2 OS shows localization to vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: beta-Glucuronidase/GUSB Antibody [NBP1-87511] - Analysis in human liver and skeletal muscle tissues using NBP1-87511 antibody. Corresponding GUSB RNA-seq data are presented for the same tissues.
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Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
VAP-B Antibody
NBP1-89112
Immunohistochemistry-Paraffin: VAP-B Antibody [NBP1-89112] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.
Immunohistochemistry-Paraffin: VAP-B Antibody [NBP1-89112] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: VAP-B Antibody [NBP1-89112] - Staining of human liver shows moderate cytoplasmic positivity in hepatocytes.
(1 Review)
(1 Publication)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, WB
RPUSD2 Antibody
NBP1-80477
Immunohistochemistry-Paraffin: RPUSD2 Antibody [NBP1-80477] - Human kidney Tissue, antibody concentration 4-8ug/ml. Cells with positive label: renal corpuscle cells (indicated with arrows) 400X magnification.
Western Blot: RPUSD2 Antibody [NBP1-80477] - Titration: 5.0ug/ml Positive Control: HepG2 cell lysate.
Species: Hu
Applications: IHC, IHC-P, WB
API5 Antibody (1C2)
H00008539-M01
Immunocytochemistry/Immunofluorescence: API5 Antibody (1C2) [H00008539-M01] - Analysis of monoclonal antibody to API5 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: API5 Antibody (1C2) [H00008539-M01] - Analysis of monoclonal antibody to API5 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.
Western Blot: API5 Antibody (1C2) [H00008539-M01] - API5 monoclonal antibody (M01), clone 1C2 Analysis of API5 expression in Hela S3 NE.
(1 Publication)
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, S-ELISA, WB
SCD-1 Antibody
[Unconjugated]
AF7550
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and SK‑Mel‑28 human malignant melanoma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human SCD‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7550) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SCD‑1 at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and SCD-1 knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human SCD‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7550) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SCD‑1 at approximately 41 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
(1 Review)
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JNK2 Antibody
(252320) [Unconjugate
MAB1846
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.2 µg/mL Mouse Anti-Human/Mouse/Rat JNK2 Monoclonal Antibody (Catalog # MAB1846) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human JNK1, JNK2, and JNK3 (1 ng/lane) were included. Specific bands for JNK2 were detected at approximately 46 and 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of HEK293T human embryonic kidney parental cell line and JNK2 knockout HEK293T cell line (KO). PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human/Mouse/Rat JNK2 Monoclonal Antibody (Catalog # MAB1846) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for JNK2 at approximately 45 and 54 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
JNK2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat JNK2 Monoclonal Antibody (Catalog # MAB1846) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
(2 Reviews)
(2 Publications)
Species: Hu, Mu, Rt
Applications: ICC, KO, WB
PTBP1 Antibody
(3H8)
H00005725-M01
Western Blot: PTBP1 Antibody (3H8) [H00005725-M01] - Analysis of PTBP1 expression in transfected 293T cell line by PTBP1 monoclonal antibody (M01), clone 3H8.Lane 1: PTBP1 transfected lysate(59.6 KDa).Lane 2: Non-transfected lysate.
Western Blot: PTBP1 Antibody (3H8) [H00005725-M01] - 2 minute exposure for Mouse embryonic stem cell - V6.5 with a dilution of 1:500 - Courtesy of Paul Fields at MIT
Western Blot: PTBP1 Antibody (3H8) [H00005725-M01] - 5 minute exposure for Mouse embryonic stem cell - V6.5 with a dilution of 1:500 - Courtesy of Paul Fields at MIT
(4 Publications)
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Applications: ELISA, ICC/IF, IHC, IHC-P, IP, Single-Cell Western, WB
VABP Antibody
NBP2-19020
Western Blot: VABP Antibody [NBP2-19020] - Analysis of VABP in (A) human brain and (B) human heart cell lysateusing this antibody at 1 ug/ml.
Species: Bv, Hu, Mu, Po, Rt
Applications: WB

BTN1A1/Butyrophilin An
NBP2-02864
Flow Cytometry: BTN1A1/Butyrophilin Antibody (OTI7B5) [NBP2-02864] - Analysis of Hela cells, using anti-BTN1A1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: BTN1A1/Butyrophilin Antibody (OTI7B5) [NBP2-02864] - Analysis of Jurkat cells, using anti-BTN1A1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: BTN1A1/Butyrophilin Antibody (OTI7B5) [NBP2-02864] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-BTN1A1 antibody, and then analyzed by flow cytometry.
Species: Ca, Hu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
MRPL39 Antibody
NBP1-86815
Immunohistochemistry-Paraffin: MRPL39 Antibody [NBP1-86815] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.
Western Blot: MRPL39 Antibody [NBP1-86815] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Western Blot: MRPL39 Antibody [NBP1-86815] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
UXT Antibody
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Western Blot: UXT Antibody [NBP1-00129] - (0.03ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: UXT Antibody [NBP1-00129] - Analysis in human thyroid Gland shows staining of cystoplasm in all epithelial cells, but nuclear staining in some of them.
(1 Publication)
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Applications: IHC, IHC-P, PEP-ELISA, WB
PLEKHG6 Antibody
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Immunohistochemistry-Paraffin: PLEKHG6 Antibody [NBP1-83840] - Staining of human placenta shows strong membranous staining positivity in trophoblastic cells.
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Applications: IHC, IHC-P
TRIM41 Antibody -
BSA Free
NBP3-05293
TRIM41 Antibody - BSA Free
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P

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Bioinformatics

Gene Symbol TRIM41