TRIM32 Antibody

Images

 
Western Blot: TRIM32 Antibody [NBP1-33737] - A. 30 ug 293T whole cell lysate/extract 7.5 % SDS-PAGETRIM32 antibody dilution: 1:1000
Immunocytochemistry/ Immunofluorescence: TRIM32 Antibody [NBP1-33737] - A375 cells were fixed in ice-cold MeOH for 5 min. Green: TRIM32 protein stained by TRIM32 antibody diluted at 1:500. Blue: Hoechst 33342 staining. ...read more
Immunohistochemistry-Paraffin: TRIM32 Antibody [NBP1-33737] - Mouse brain diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: TRIM32 Antibody [NBP1-33737] - A. 5 ug 293T whole cell lysate/extract B. 5 ug whole cell lysate/extract of DDDDK-human TRIM32-transfected 293T cells 7.5 % SDS-PAGE TRIM32 antibody dilution: 1:20000

Product Details

Summary
Reactivity Hu, Mu, Rt, Fe, RMSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

TRIM32 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human TRIM32. The exact sequence is proprietary.
Localization
Nucleus
Predicted Species
Rat (98%), Rhesus Macaque (99%), Feline (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TRIM32
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:20000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
72 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP1-33737 in the following applications:

  • 1 publication
  • WB
    1 publication

Reactivity Notes

Expected cross reactivity based on sequence homology: Cat (98%), Bovine (88%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for TRIM32 Antibody

  • 72 kDa Tat-interacting protein
  • BBS11
  • BBS1172-KD
  • EC 6.3.2.-
  • HT2A
  • HT2Alimb girdle muscular dystrophy 2H (autosomal recessive)
  • LGMD2H
  • TATIP
  • TRIM32
  • tripartite motif containing 32
  • Zinc finger protein HT2A
  • zinc-finger protein HT2A

Background

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies. The protein has also been localized to the nucleus, where it interacts with the activation domain of the HIV-1 Tat protein. The Tat protein activates transcription of HIV-1 genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for TRIM32 Antibody (NBP1-33737)(2)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: IHC-P, WB.


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IHC-P
(1)
WB
(1)
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(1)
Mouse
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol TRIM32