TRIM25 Antibody Blocking Peptide

Western Blot: TRIM25 Peptide [NBP1-76825PEP] - blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for 30 min at 37C.

• Reactivity: Hu
• Applications: AC
• Concentration: 0.2 mg/ml

TRIM25 Antibody Blocking Peptide Summary

• Description: A blocking peptide containing 19 amino acids near the carboxy terminus of human TRIM25.

  Source: Synthetic

  (Accession #: NP_005073)

• Source: Synthetic
• Protein/Peptide Type: Antibody Blocking Peptide
• Gene: TRIM25
• Purity: N/A

Applications/Dilutions

• Dilutions:
  • Antibody Competition
• Application Notes: This peptide is useful as a blocking peptide for NBP1-76825.
  
  For further blocking peptide related protocol, click here.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS pH 7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin.
• Preservative: 0.02% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: N/A

Alternate Names for TRIM25 Antibody Blocking Peptide

• EC 6.3.2.19
• EC 6.3.2.n3
• EFP
• EFPZ147
• Estrogen-responsive finger protein
• RING finger protein 147
• RNF147
• RNF147E3 ubiquitin/ISG15 ligase TRIM25
• TRIM25
• tripartite motif containing 25
• tripartite motif-containing 25
• Tripartite motif-containing protein 25
• Ubiquitin/ISG15-conjugating enzyme TRIM25
• zinc finger protein 147 (estrogen-responsive finger protein)
• Zinc finger protein 147
• zinc finger protein-147
• ZNF147
• ZNF147tripartite motif protein TRIM25

Background

TRIM25 is encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: TRIM25